FastQCFastQC Report
Wed 9 Nov 2016
test1.R1.txt.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenametest1.R1.txt.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10000
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTAA652.1014333E-531.84615369
GAGACTA901.9288648E-423.068
CGAGACT1304.1680214E-621.2307769
ACGAGAC1602.5068064E-517.2568
CACGAGA1755.4099008E-515.77142967
CCACGAG1806.8849724E-515.33333366
CCCACGA1901.09254295E-414.52631565
GCCCACG1951.3632367E-414.15384764
CGAGCCC2354.291479E-513.21276661
AGCCCAC2153.1233806E-412.8372163
GAGCCCA2305.5213826E-412.062
CCGAGCC2500.001112723811.0460
Produced by FastQC (version 0.11.3)