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# Project description

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Automated Feedback Procedures for the Fly Embryo Screen.
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# Experiment setup

Multichamber wide slide with fixed fly embryos stained with 3 dyes:
- DAPI: nuclei
- Alexa488: antibody binds to Ds-red
- Alexa633: antibody for LacZ.

# Feedback microscopy protocol:
1. Select manually positions corresponding to the centers of the wells
2. Tile scan overview image with 5x objective.
3. Finding positions of embryos via feedback analysis
4. ...

# Log/updates
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2018-01-05 Tim and Alex
- Configuring acquisition jobs manually.
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- Trying feedback experiments with manual selection procedures
- X-Y coordinates are flipping, microscope additionally scans from right to left