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ALMF
feedback-fly-embryo-crocker
Commits
bef95d25
Commit
bef95d25
authored
Sep 26, 2018
by
Aliaksandr Halavatyi
Browse files
Autofocus with offset job moved to AutoMicTools
parent
0826c080
Changes
3
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Inline
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src/main/java/feedback/fly/embryo/jobdistributors/JobDistributor_AutoSelectionMultiple_AFocus.java
View file @
bef95d25
...
...
@@ -7,12 +7,12 @@ import ij.gui.WaitForUserDialog;
import
ij.plugin.PlugIn
;
import
automic.online.jobdistributors.JobDistributor_Abstract
;
import
automic.online.jobs.common.Job_AutofocusInitOffset
;
import
automic.online.jobs.common.Job_RecordFinish
;
import
automic.parameters.ParameterCollection
;
import
automic.parameters.gui.ParameterGuiManager
;
import
automic.table.TableModel
;
import
automic.table.TableProcessor
;
import
feedback.fly.embryo.jobs.Job_AutofocusInitOffset
;
import
feedback.fly.embryo.jobs.Job_FocusEmbryoAuto
;
import
feedback.fly.embryo.jobs.Job_SelectMultipleEmbryosAuto
;
...
...
src/main/java/feedback/fly/embryo/jobdistributors/JobDistributor_AutoSelectionMultiple_AFocus_Rotation.java
View file @
bef95d25
...
...
@@ -7,12 +7,12 @@ import ij.gui.WaitForUserDialog;
import
ij.plugin.PlugIn
;
import
automic.online.jobdistributors.JobDistributor_Abstract
;
import
automic.online.jobs.common.Job_AutofocusInitOffset
;
import
automic.online.jobs.common.Job_RecordFinish
;
import
automic.parameters.ParameterCollection
;
import
automic.parameters.gui.ParameterGuiManager
;
import
automic.table.TableModel
;
import
automic.table.TableProcessor
;
import
feedback.fly.embryo.jobs.Job_AutofocusInitOffset
;
import
feedback.fly.embryo.jobs.Job_FocusEmbryoAutoRotation
;
import
feedback.fly.embryo.jobs.Job_SelectMultipleEmbryosAutoRotation
;
...
...
src/main/java/feedback/fly/embryo/jobs/Job_AutofocusInitOffset.java
deleted
100644 → 0
View file @
0826c080
package
feedback.fly.embryo.jobs
;
import
java.awt.Color
;
import
automic.online.microscope.ZeissKeys
;
import
automic.online.jobs.Job_Default
;
import
automic.parameters.ParameterCollection
;
import
automic.parameters.ParameterType
;
import
automic.utils.imagefiles.ImageOpenerWithBioformats
;
import
ij.IJ
;
import
ij.ImageJ
;
import
ij.ImagePlus
;
import
ij.gui.Line
;
import
ij.gui.Overlay
;
import
ij.gui.ProfilePlot
;
import
ij.gui.Roi
;
import
ij.plugin.MontageMaker
;
public
class
Job_AutofocusInitOffset
extends
Job_Default
{
public
static
final
String
KEY_FOCUS_SLICE_OFFSET
=
"Slice offset"
;
int
sliceOffset
=
2000
;
//private static final Roi nullRoi=null;
private
ImagePlus
img
=
null
;
int
maxind
=-
1
;
Roi
ln
=
null
;
@Override
protected
void
cleanIterOutput
(){
img
=
null
;
ln
=
null
;
}
@Override
protected
void
preProcessOnline
()
throws
Exception
{
super
.
clearSharedData
();
currentTable
.
cleanRecord
(
curDInd
);
currentTable
.
setFileAbsolutePath
(
newImgFile
,
curDInd
,
imgColumnNm
,
"IMG"
);
String
Exper_nm
=
newImgFile
.
getName
();
Exper_nm
=
Exper_nm
.
substring
(
0
,
Exper_nm
.
indexOf
(
fileTag
));
this
.
setSharedValue
(
"Experiment Name"
,
Exper_nm
);
img
=
ImageOpenerWithBioformats
.
openImage
(
newImgFile
);
img
=
new
MontageMaker
().
makeMontage2
(
img
,
1
,
img
.
getNSlices
(),
1.0
,
1
,
img
.
getNSlices
(),
1
,
0
,
false
);
}
@Override
protected
void
preProcessOffline
()
throws
Exception
{
img
=
ImageOpenerWithBioformats
.
openImage
(
currentTable
.
getFile
(
curDInd
,
imgColumnNm
,
"IMG"
));
img
=
new
MontageMaker
().
makeMontage2
(
img
,
1
,
img
.
getNSlices
(),
1.0
,
1
,
img
.
getNSlices
(),
1
,
0
,
false
);
}
@Override
protected
boolean
runProcessing
()
throws
Exception
{
//find focus and send to the microscope macro
img
.
setRoi
(
0
,
0
,
img
.
getWidth
(),
img
.
getHeight
());
double
[]
vals
=
new
ProfilePlot
(
img
,
true
).
getProfile
();
img
.
setRoi
((
Roi
)
null
);
int
npoints
=
vals
.
length
;
double
maxv
=
0
;
maxind
=-
1
;
for
(
int
i
=
0
;
i
<
npoints
;
i
++){
if
(
vals
[
i
]>
maxv
){
maxind
=
i
;
maxv
=
vals
[
i
];
}
}
IJ
.
log
(
"maxind: "
+
maxind
);
ln
=
new
Line
(
0
,
maxind
,
img
.
getWidth
(),
maxind
);
ln
.
setStrokeColor
(
Color
.
ORANGE
);
return
true
;
}
@Override
protected
Overlay
createOverlay
(){
return
(
ln
!=
null
)?
new
Overlay
(
ln
):
new
Overlay
();
}
@Override
public
void
visualise
(
int
_xvis
,
int
_yvis
){
this
.
visualiseImg
(
img
,
getOverlay
(),
_xvis
,
_yvis
);
}
@Override
public
void
postProcessSuccess
()
throws
Exception
{
ZeissKeys
.
submitCommandsToMicroscope
(
"focus"
,
""
+(
img
.
getWidth
()-
1
)/
2.0
,
"0"
,
""
+(
maxind
+
sliceOffset
),
""
,
""
,
""
,
""
,
""
);
saveRoiForImage
(
newImgFile
,
ln
);
}
@Override
public
ParameterCollection
createJobParameters
(){
ParameterCollection
jobCollection
=
new
ParameterCollection
();
jobCollection
.
addParameter
(
KEY_FOCUS_SLICE_OFFSET
,
null
,
sliceOffset
,
ParameterType
.
INT_PARAMETER
);
return
jobCollection
;
}
@Override
public
void
parseInputParameterValues
(
ParameterCollection
_jobParameterCollection
){
this
.
sliceOffset
=(
Integer
)
_jobParameterCollection
.
getParameterValue
(
KEY_FOCUS_SLICE_OFFSET
);
}
/**
* offline debugging
* @param args unsused
*/
public
static
void
main
(
String
[]
args
)
throws
Exception
{
// start ImageJ
new
ImageJ
();
String
tblPth
=
"D:/tempDat/Crocker_group/fly feedback files 20180410/ttt"
;
String
tblFnm
=
"summary_test05_.txt"
;
Job_AutofocusInitOffset
testJob
=
new
Job_AutofocusInitOffset
();
testJob
.
initialise
(
null
,
"AFocus"
,
false
);
testJob
.
testJobMicTable
(
0
,
tblPth
,
tblFnm
);
System
.
out
.
print
(
"test end"
);
}
}
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