Commit ae1e149d authored by Christian Tischer's avatar Christian Tischer

EMBL Course

parent ad0d771b
...@@ -22,26 +22,30 @@ digraph G { ...@@ -22,26 +22,30 @@ digraph G {
image -> "large blur"; image -> "large blur";
"small blur" -> "noise filtered"; "small blur" -> "noise filtered";
"large blur" -> "local background"; "large blur" -> "local background";
"small blur" -> "DoG = small blur - large blur"; "small blur" -> "small blur - large blur" -> "DoG";
"large blur" -> "DoG = small blur - large blur"; "large blur" -> "small blur - large blur" -> "DoG";
"DoG" -> "Laplacian of Gaussian (LoG)" [label=" is related"];
} }
'/> '/>
### Activity: Enhance spots in noisy image with uneven background ### Activity: Enhance spots in noisy image with uneven background
- Open image: xy_8bit__two_spots_noisy_uneven_background.tif - Open image: xy_8bit__two_spots_noisy_uneven_background.tif
- Appreciate that you cannot simply threshold them - Appreciate that you cannot readily threshold the spots
- Copy image and blur with a Gaussian of small sigma -> Gs - Compute DoG:
- Copy image and blur with a Gaussian of bigger sigma -> Gb - Copy image and blur with a Gaussian of small sigma -> Gs
- For the official DoG: `rb = sqrt(2) * rs` - Copy image and blur with a Gaussian of bigger sigma -> Gb
- Create `DoG = Gs - Gb` - For the official DoG: `rb = sqrt(2) * rs`
- Appreciate that you can simply threshold the DoG image - Create `DoG = Gs - Gb`
- Appreciate that now it is possible to threshold the spots in the DoG image
### Learn more
- https://en.wikipedia.org/wiki/Difference_of_Gaussians
### Formative Assessment ### Formative Assessment
TODO TODO
### Learn more
- https://en.wikipedia.org/wiki/Difference_of_Gaussians
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