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image-analysis-training-resources
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grp-bio-it
image-analysis-training-resources
Commits
d8edc0f6
Commit
d8edc0f6
authored
Apr 05, 2019
by
Christian Tischer
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workshops/.bioimage-analysis-fundamentals.md.swp
workshops/.bioimage-analysis-fundamentals.md.swp
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workshops/image-feature-enhancement.md
workshops/image-feature-enhancement.md
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workshops/machine-learning-semantic-image-segmentation.md
workshops/machine-learning-semantic-image-segmentation.md
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workshops/rank-filters.md
workshops/rank-filters.md
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workshops/.bioimage-analysis-fundamentals.md.swp
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workshops/image-feature-enhancement.md
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d8edc0f6
# Image feature enhancement
<img src='https://g.gravizo.com/svg?
digraph G {
shift [fontcolor=white,color=white];
image -> filter -> "enhanced image";
"enhanced image" -> "feature" [label=" aka"];
filter -> "feature enhancement" [label=" aka"];
}
'/>
## Difference of Gaussian (DoG) for spot enhancement
<img src='https://g.gravizo.com/svg?
digraph G {
shift [fontcolor=white,color=white];
image -> "small blur";
image -> "large blur";
"small blur" -> "remove noise";
"large blur" -> "estimate local background";
"small blur" -> "DoG = small blur - large blur";
"large blur" -> "DoG = small blur - large blur";
}
'/>
### Activity: Enhance spots in noisy image with uneven background
-
Open image: xy_8bit__two_spots_noisy_uneven_background.tif
-
Appreciate that you cannot simply threshold them
-
Copy the image and blur with a Gaussian of small radius -> gs
-
Copy the image and blur with a Gaussian of bigger radius -> gb
-
Create
`DoG = gs - gb`
-
Appreciate that you can simply threshold the DoG image
### Formative Assessment
TODO
### Learn more
-
https://en.wikipedia.org/wiki/Difference_of_Gaussians
workshops/machine-learning-semantic-image-segmentation.md
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d8edc0f6
...
...
@@ -13,7 +13,7 @@
"pixel class image" -> "class00 value";
"pixel class image" -> "class01 value";
"pixel class image" -> "class.. value";
"pixel class image" -> "class
N
value";
"pixel class image" -> "class
C
value";
}
'/>
...
...
workshops/rank-filters.md
0 → 100644
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d8edc0f6
# Rank filters
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