Commit f42a00d5 authored by Christian Tischer's avatar Christian Tischer

Update image-ethics-and-data-integrity.md

parent 764c31cf
...@@ -43,7 +43,7 @@ Repeat above activity ...@@ -43,7 +43,7 @@ Repeat above activity
- testing different formats for saving - testing different formats for saving
- adjusting the image display before saving - adjusting the image display before saving
### Formative Assessments ### Formative assessments
How can I ensure that image content is preserved during saving (one answer)? How can I ensure that image content is preserved during saving (one answer)?
...@@ -74,6 +74,8 @@ How can I ensure that image content is preserved during saving (one answer)? ...@@ -74,6 +74,8 @@ How can I ensure that image content is preserved during saving (one answer)?
### Activities ### Activities
#### Comparing images
- Open image: xy_calibrated_16bit__nuclear_protein_control.tif - Open image: xy_calibrated_16bit__nuclear_protein_control.tif
- This image shows a nuclear protein in control cells. - This image shows a nuclear protein in control cells.
- Open image: xy_calibrated_16bit__nuclear_protein_treated.tif - Open image: xy_calibrated_16bit__nuclear_protein_treated.tif
...@@ -83,15 +85,18 @@ How can I ensure that image content is preserved during saving (one answer)? ...@@ -83,15 +85,18 @@ How can I ensure that image content is preserved during saving (one answer)?
- Adjust the lookup-tables (LUTs) of both images to be the same - Adjust the lookup-tables (LUTs) of both images to be the same
- Add the LUT calibration to both images - Add the LUT calibration to both images
#### Adjusting the LUT for high dynamic range images
### Formative Assessments ### Formative Assessments
What helps to scientifically convey image intensity information (multiple answers)? What helps to scientifically convey image intensity information (multiple answers)?
1. Use a grayscale LUT whenever possible 1. Use a grayscale LUT whenever possible.
2. Color the images in the color of the fluorophore 2. Color the images in the color of the fluorophore.
3. Add a LUT calibration bar 3. Add a LUT calibration bar.
4. Use the same LUT for all images 4. Use the same LUT for all images.
5. Adjust the LUT to the full bit-depth of the images 5. Always adjust the LUT to the image's full bit-depth.
6. Never change the LUT of images! Always keep it as in the raw data. 6. Never change the LUT of images! Always keep it as in the raw data.
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