In this WiKi we provide examples on how to analyze and process FCS and imaging data generated on Zeiss LSM microscopes using FCSRunner or MyPiC and Automated FCS. The processed data can be used to generate a FCS calibration curve for converting pixel fluorescence intensities in to concentrations and protein numbers.
The processing steps are
IIIb. Fit F(C)CS data with Fluctuation Analyzer 4G (FA) (alternative to 3a)
Antonio Politi, EMBL, July 2017