Skip to content
GitLab
Projects Groups Snippets
  • /
  • Help
    • Help
    • Support
    • Community forum
    • Submit feedback
    • Contribute to GitLab
  • Sign in
  • mypic mypic
  • Project information
    • Project information
    • Activity
    • Labels
    • Members
  • Repository
    • Repository
    • Files
    • Commits
    • Branches
    • Tags
    • Contributors
    • Graph
    • Compare
  • Issues 7
    • Issues 7
    • List
    • Boards
    • Service Desk
    • Milestones
  • Deployments
    • Deployments
    • Releases
  • Packages and registries
    • Packages and registries
    • Container Registry
  • Monitor
    • Monitor
    • Incidents
  • Analytics
    • Analytics
    • Value stream
    • Repository
  • Wiki
    • Wiki
  • Activity
  • Graph
  • Create a new issue
  • Commits
  • Issue Boards
Collapse sidebar
  • Ellenberg GroupEllenberg Group
  • mypicmypic
  • Wiki
  • Home

Home · Changes

Page history
Short description of tracking pipeline authored May 28, 2018 by Antonio Politi's avatar Antonio Politi
Hide whitespace changes
Inline Side-by-side
Home.md
View page @ 4bff5441
# Microscopy Pipeline Constructor (MyPiC)
MyPiC is a Visual Basic for Application (VBA) macro for Zeiss confocal microscopes running with the ZEN software (version black >= ZEN2010). The macro has been developed in the group of Jan Ellenberg, EMBL, Heidelberg, and replaces the AutofocusScreenMacro.
MyPiC is a Visual Basic for Application (VBA) macro for Zeiss confocal microscopes running with the ZEN software (version black >= ZEN2010). The macro has been developed in the group of Jan Ellenberg, EMBL, Heidelberg, and replaces the AutofocusScreenMacro.
The macro allows
......@@ -82,7 +82,7 @@ An example workflow using glass reflection autofocus or fluorescence based autof
* Glass based reflection and imaging
* Fluorescence intensity center of mass based tracking
The interaction with the adaptive feedback microscopy is exaplaied in
The interaction with the adaptive feedback microscopy is explained in
* [Adaptive feedback microscopy/Online image analysis](./Adaptivefeedback)
......@@ -138,6 +138,9 @@ For each imaging and FCS [task](#task) several options can be specified. These d
For imaging tasks the user can specify further processing steps. For instance, the macro can compute a new XYZ position from the center of mass of the fluorescence image. The current reference position can then be updated with the computed new position (options **TrackXY** and/or **TrackZ** are on). This feature is useful to track bright objects in 3D. More complicated processing steps can be achieved when an external image analysis program is used (see also [Adaptive feedback microscopy/Online image analysis](#adaptivefeedback)).
> The computations `Center of mass` and `Center of mass (thr)` within MyPiC do not work with images acquired with the **Airy Detector**. To track objects using the **Airy detector** acquired images refer to the [Adaptive feedback microscopy/Online image analysis](#adaptivefeedback).
**Task options**
<p align= center> <img src="./images/pipcon_track.PNG" width = "400px"> <p/>
......
Clone repository
  • AdaptiveFeedback
  • Home