#!/usr/bin/env python3
import argparse
from pathlib import Path
from tempfile import TemporaryDirectory
import matplotlib.pyplot as plt
import pandas as pd
import streamlit as st
import plotting_on_genome as pog
# st.set_page_config(layout="wide")
if "stage" not in st.session_state:
st.session_state.stage = 0
if "insert_types" not in st.session_state:
st.session_state.insert_types = "both"
if "workdir" not in st.session_state:
st.session_state.workdir = None
class TempDirManager:
def __init__(self, dirpath=None):
self.dirpath = dirpath
def __enter__(self):
if self.dirpath is None:
self.temp_dir = TemporaryDirectory()
return self.temp_dir.name
else:
Path(self.dirpath).mkdir(exist_ok=True)
return self.dirpath
def __exit__(self, exc_type, exc_value, traceback):
if self.dirpath is None:
self.temp_dir.cleanup()
@st.cache_data
def run_pipeline(
seq_fh, genome_fh, email, search_term, retmax, fwd_suf, rev_suf, workdir=None
):
with st.spinner("Processing..."), TempDirManager(workdir) as work_dir:
dirpath = Path(work_dir)
if genome_fh is not None:
genome_path = str(dirpath / genome_fh.name)
with open(genome_path, "wb") as fh:
fh.write(genome_fh.getvalue())
else:
genome_path = None
seq_path = str(dirpath / "seqs.fasta")
with open(seq_path, "wb") as fh:
for seq in seq_fh:
# Make sure that there is new line between individual seqs
fh.write(seq.getvalue())
fh.write(b"\n")
res = pog.InsertsDict(
seq_file=seq_path,
work_dir=dirpath,
genome_file=genome_path,
search_term=search_term,
email=email,
retmax=retmax,
fwd_suffix=fwd_suf,
rev_suffix=rev_suf,
)
return res
def submit(*args):
st.session_state.pipeline = run_pipeline(*args)
st.session_state.stage = 1
def get_main_inputs():
parser = argparse.ArgumentParser("PlottingOnGenome")
parser.add_argument("--workdir", action="store_true")
args = parser.parse_args()
# Radio buttons to switch between text input and file upload
genome_src = st.radio("Genome:", ("NCBI", "File"))
# Text inputs
genome_fh = None
search_term = None
email = None
retmax = None
if genome_src == "NCBI":
search_term = st.text_input("Search term:", key="search_term")
email = st.text_input("Email:", key="email")
retmax = st.text_input(
"Retmax (Max. # of records from NCBI)", 200, key="retmax"
)
else:
genome_fh = st.file_uploader(
"Upload genome:", type=("gbk", "gff"), key="genome"
)
seq_fh = st.file_uploader(
"Sequence file:",
type=["fasta", "fas", "fna"],
key="seqs",
accept_multiple_files=True,
)
fwd_suf = st.text_input("Forward suffix:", "_F", key="fwd_suf")
rev_suf = st.text_input("Reverse suffix:", "_R", key="rev_suf")
st.session_state.insert_types = st.selectbox(
"insert types:", ["both", "matched", "unmatched"]
)
if args.workdir:
st.session_state.workdir = st.text_input("workdir", "Output")
st.button(
"Submit",
on_click=submit,
args=[
seq_fh,
genome_fh,
email,
search_term,
retmax,
fwd_suf,
rev_suf,
st.session_state.workdir,
],
)
def show_results():
option = st.selectbox(
"plot type:",
["plot inserts", "plot genome", "plot insert dists"],
None,
)
inserts_all = st.session_state.pipeline
insert_types = st.session_state.insert_types
filter_threshold = st.slider("Filter threshold:", 0.0, 1.0, 0.7)
if inserts_all is not None:
if option == "plot inserts":
buffer = st.slider(
"View window size:",
min_value=0,
max_value=10000,
value=4000,
help="Number of bases either side of the insert",
)
st.write("Features to dispaly:")
ft_checkboxes = {
"CDS": st.checkbox("CDS", value=True),
"gene": st.checkbox("gene", value=True),
}
feature_types = [k for k, v in ft_checkboxes.items() if v]
seq_id = st.selectbox("Select sequence id:", inserts_all.seq_ids)
inserts = inserts_all.get(seq_id, insert_types, filter_threshold)
if len(inserts):
df_features = pd.concat(
[
insert.to_dataframe().assign(insert_idx=idx + 1)
for idx, insert in enumerate(inserts)
]
).reset_index(drop=True)
st.write(df_features)
for idx, insert in enumerate(inserts):
fig, axs = plt.subplots(
2, 1, figsize=(10, 10), height_ratios=[3, 5]
)
fig.suptitle(f"Insert {idx+1}")
axs = insert.plot(
buffer=buffer, axs=axs, feature_types=feature_types
)
st.pyplot(fig)
plt.close()
else:
st.write(f"No inserts found for {seq_id}!")
elif option == "plot genome":
labels = st.toggle("Labels")
inserts = []
if st.toggle("Plot inserts", True):
inserts = inserts_all.filter(insert_types, filter_threshold)
st.write(inserts_all.to_dataframe(insert_types, filter_threshold))
fig, ax = plt.subplots(figsize=(10, 10 * (1 + 2 * labels)))
ax = inserts_all.plot(inserts, show_labels=labels, ax=ax)
st.pyplot(fig, use_container_width=True)
plt.close()
elif option == "plot insert dists":
plot_type = st.radio(
"Plot type:", ["histogram", "violinplot+boxplot+stripplot"]
)
inserts = inserts_all.filter(insert_types, filter_threshold)
if plot_type == "histogram":
fig, axs = plt.subplots(1, 2, figsize=(12, 5))
pog.plot_histogram(inserts, axs=axs)
st.pyplot(fig)
elif plot_type == "violinplot+boxplot+stripplot":
fig, axs = plt.subplots(1, 2, figsize=(12, 5))
pog.plot_dists(inserts, axs=axs)
st.pyplot(fig)
else:
raise ValueError("Incorrect plot type")
plt.close()
def main():
col1, col2 = st.columns((6, 1), gap="large", vertical_alignment="bottom")
with col1:
st.title("PlottingOnGenome")
with col2:
if st.session_state.stage == 1:
if st.button("Reset"):
st.session_state.stage = 0
st.rerun()
if st.session_state.stage == 0:
get_main_inputs()
if st.session_state.stage >= 1:
show_results()
if __name__ == "__main__":
main()