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script-and-macro-examples/Segment_nuclei.ijm

+//rename("origin");
+//closeAllExceptOrigin()
+run("Set Measurements...", "area mean centroid center display redirect=None decimal=6");
+run("Set Scale...", "distance=0 known=0 unit=pixel");
+run("Duplicate...", "duplicate channels=1");
+run("Gaussian Blur...", "sigma=2");
+setAutoThreshold("Default dark");
+roiManager("reset");
+//run("Analyze Particles...", "size=100-Infinity pixel display clear include add");
+run("Analyze Particles...", "size=2000-Infinity pixel display clear include add exclude");
+run("Set Scale...", "distance=0 known=0 unit=pixel");
+run("Set Measurements...", "area mean centroid center display redirect=None decimal=6");
+//run("Analyze Particles...", "size=100-Infinity display clear include add");