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LocScale is a reference-based local amplitude scaling tool using prior model information to improve contrast of cryo-EM density maps. It can be used as an alternative to other commonly used map sharpening methods.
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To reliably reflect the required level of local sharpening and local resolution variation in the target map, atomic B-factors of the reference model should have been refined using appropriate protocols prior to scaling. The method is described in more detail here [1](https://doi.org/10.7554/eLife.27131).
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To reliably reflect the required level of local sharpening and local resolution variation in the target map, atomic B-factors of the reference model should have been refined using appropriate protocols prior to scaling. The method is described in more detail here [[1]](https://doi.org/10.7554/eLife.27131).
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