diff --git a/pyfastani/_fastani.pyx b/pyfastani/_fastani.pyx
index 84b348230fa3977c20fe7e4a28e57a8ff6b9df3b..456d0c6872075d48ece8eb0fbb910dcef74748eb 100644
--- a/pyfastani/_fastani.pyx
+++ b/pyfastani/_fastani.pyx
@@ -15,10 +15,11 @@ References:
cimport cython
cimport cython.parallel
cimport libcpp11.chrono
+from cpython.buffer cimport Py_buffer, PyBUF_READ
from cython.operator cimport dereference, preincrement, postincrement
from cpython.ref cimport Py_INCREF
from cpython.list cimport PyList_New, PyList_SET_ITEM
-from cpython.pycapsule cimport PyCapsule_New, PyCapsule_GetPointer, PyCapsule_CheckExact
+from cpython.memoryview cimport PyMemoryView_FromMemory, PyMemoryView_Check, PyMemoryView_GET_BUFFER
from libc.stdio cimport printf
from libc.string cimport memcpy
from libc.limits cimport INT_MAX
@@ -821,8 +822,6 @@ cdef class Mapper(_Parameterized):
@staticmethod
cdef void _do_l1_mappings(
- const Parameters_t& param,
- const Sketch_t& ref_sketch,
Map_t& map,
const int kind,
const void* data,
@@ -843,14 +842,14 @@ cdef class Mapper(_Parameterized):
cdef MinimizerMapValueType_t hit_position_list
# compute minimizers
- if param.alphabetSize == 4:
+ if map.param.alphabetSize == 4:
_add_minimizers_nucl(
query.minimizerTableQuery,
kind,
data,
slen,
- param.kmerSize,
- param.windowSize,
+ map.param.kmerSize,
+ map.param.windowSize,
0,
)
else:
@@ -859,8 +858,8 @@ cdef class Mapper(_Parameterized):
kind,
data,
slen,
- param.kmerSize,
- param.windowSize,
+ map.param.kmerSize,
+ map.param.windowSize,
0,
)
@@ -879,15 +878,15 @@ cdef class Mapper(_Parameterized):
# keep minimizer if it exist in the reference lookup index
it = query.minimizerTableQuery.begin()
while it != uniq_end_iter:
- seed_find = ref_sketch.minimizerPosLookupIndex.const_find(dereference(it).hash)
- if seed_find != ref_sketch.minimizerPosLookupIndex.end():
+ seed_find = map.refSketch.minimizerPosLookupIndex.const_find(dereference(it).hash)
+ if seed_find != map.refSketch.minimizerPosLookupIndex.end():
hit_position_list = dereference(seed_find).second
- if hit_position_list.size() < ref_sketch.getFreqThreshold():
+ if hit_position_list.size() < map.refSketch.getFreqThreshold():
seed_hits_l1.insert(seed_hits_l1.end(), hit_position_list.begin(), hit_position_list.end())
postincrement(it)
# estimate the number of minimum hits, and compute candidates
- minimum_hits = estimateMinimumHitsRelaxed(query.sketchSize, param.kmerSize, param.percentageIdentity)
+ minimum_hits = estimateMinimumHitsRelaxed(query.sketchSize, map.param.kmerSize, map.param.percentageIdentity)
map.computeL1CandidateRegions(query, seed_hits_l1, minimum_hits, l1_mappings)
cpdef void _query_fragment(
@@ -895,17 +894,26 @@ cdef class Mapper(_Parameterized):
_Map map,
const int i,
const seqno_t seq_counter,
- const unsigned char[::1] sequence,
+ object mem,
+ int kind,
+ int stride,
_FinalMappings final_mappings,
) except *:
cdef kseq_t kseq
cdef QueryMetaData_t[kseq_ptr_t, Map_t.MinVec_Type] query
cdef vector[L1_candidateLocus_t] l1_mappings
- cdef const unsigned char* fragment
+ cdef Py_buffer* buffer
+ cdef char* data
+ cdef char* fragment
- with nogil:
+ if __debug__:
+ if not PyMemoryView_Check(mem):
+ raise TypeError(f"expected memoryview, got {type(mem).__name__!r}")
+ buffer = PyMemoryView_GET_BUFFER(mem)
- fragment = &sequence[i*self._param.minReadLength]
+ with nogil:
+ data = <char*> buffer.buf
+ fragment = &data[i*self._param.minReadLength*stride]
query.kseq = &kseq
query.kseq.seq.s = NULL
@@ -913,12 +921,9 @@ cdef class Mapper(_Parameterized):
query.seqCounter = seq_counter + i
Mapper._do_l1_mappings(
- # classes with configuration
- self._param,
- self._sk[0],
map._map[0],
# start of the i-th fragment
- PyUnicode_1BYTE_KIND,
+ kind,
fragment,
self._param.minReadLength,
# outputs
@@ -926,7 +931,11 @@ cdef class Mapper(_Parameterized):
l1_mappings,
)
- map._map.doL2Mapping(query, l1_mappings, final_mappings._vec)
+ map._map.doL2Mapping(
+ query,
+ l1_mappings,
+ final_mappings._vec
+ )
cdef list _query_draft(self, object contigs):
"""Query the sketcher for the given contigs.
@@ -943,11 +952,16 @@ cdef class Mapper(_Parameterized):
# bookkeeping
cdef uint64_t total_fragments = 0
cdef uint64_t total_length = 0
- # mapping parameters and reference
+ # compute map and L2 mappings
cdef _Map map
cdef _FinalMappings final_mappings
# sequence as a unicode object
+ cdef const unsigned char[::1] view
+ cdef int kind
+ cdef int stride
+ cdef void* data
cdef ssize_t slen
+ cdef object mem
# core genomic identity results
cdef vector[CGI_Results] results
cdef CGI_Results result
@@ -956,21 +970,14 @@ cdef class Mapper(_Parameterized):
cdef uint64_t shared_length
cdef list hits = []
- cdef object l2_lock = threading.Lock()
-
- # create a new mapper with the given mapping result vector
+ # create a new mapper and a new l2 mapping vector
map = _Map.__new__(_Map)
map._map = new Map_t(self._param, self._sk[0], total_fragments, 0)
-
- # create the result vector
final_mappings = _FinalMappings.__new__(_FinalMappings)
- #
+ # spawn a thread pool to map fragments in parallel for all the contigs
with multiprocessing.pool.ThreadPool() as pool:
-
- # iterate over contigs
for contig in contigs:
-
# check length of contig is enough for computing mapping
slen = len(contig)
if slen < min(self._param.windowSize, self._param.kmerSize, self._param.minReadLength):
@@ -981,65 +988,46 @@ cdef class Mapper(_Parameterized):
),
UserWarning,
)
-
- # encode
+ # get a way to read each letter of the contig,
+ # independently of it being `str`, `bytes`, `bytearray`, etc.
if isinstance(contig, str):
- contig = contig.encode('ascii')
-
- # # get a way to read each letter of the contig,
- # # independently of it being `str`, `bytes`, `bytearray`, etc.
- # if isinstance(contig, str):
- # # make sure the unicode string is in canonical form,
- # # --> won't be needed anymore in Python 3.12
- # IF SYS_VERSION_INFO_MAJOR <= 3 and SYS_VERSION_INFO_MINOR < 12:
- # PyUnicode_READY(contig)
- # # get kind and data for efficient indexing
- # kind = PyUnicode_KIND(contig)
- # data = PyUnicode_DATA(contig)
- # slen = PyUnicode_GET_LENGTH(contig)
- # if kind == PyUnicode_1BYTE_KIND:
- # stride = sizeof(Py_UCS1)
- # elif kind == PyUnicode_2BYTE_KIND:
- # stride = sizeof(Py_UCS2)
- # else:
- # stride = sizeof(Py_UCS4)
- # else:
- # # attempt to view the contig as a buffer of contiguous bytes
- # view = contig
- # # pretend the bytes are an ASCII (UCS-1) encoded string
- # kind = PyUnicode_1BYTE_KIND
- # slen = view.shape[0]
- # stride = sizeof(Py_UCS1)
- # if slen != 0:
- # data = <void*> &view[0]
-
+ # make sure the unicode string is in canonical form,
+ # --> won't be needed anymore in Python 3.12
+ IF SYS_VERSION_INFO_MAJOR <= 3 and SYS_VERSION_INFO_MINOR < 12:
+ PyUnicode_READY(contig)
+ # get kind and data for efficient indexing
+ kind = PyUnicode_KIND(contig)
+ data = PyUnicode_DATA(contig)
+ slen = PyUnicode_GET_LENGTH(contig)
+ if kind == PyUnicode_1BYTE_KIND:
+ stride = sizeof(Py_UCS1)
+ elif kind == PyUnicode_2BYTE_KIND:
+ stride = sizeof(Py_UCS2)
+ else:
+ stride = sizeof(Py_UCS4)
+ else:
+ # attempt to view the contig as a buffer of contiguous bytes
+ view = contig
+ # pretend the bytes are an ASCII (UCS-1) encoded string
+ kind = PyUnicode_1BYTE_KIND
+ slen = view.shape[0]
+ stride = sizeof(Py_UCS1)
+ if slen != 0:
+ data = <void*> &view[0]
+ # turn the void pointer into a Python object so it can
+ # be passed to another Python method
+ mem = PyMemoryView_FromMemory(<char*> data, slen*stride, PyBUF_READ)
# compute the expected number of blocks
fragment_count = slen // self._param.minReadLength
-
- # map the blocks
- # for i in range(fragment_count):
- # self._query_fragment(
- # map,
- # # position in query
- # i,
- # total_fragments,
- # # sequence
- # contig,
- # # result vector
- # final_mappings
- # )
+ # map the blocks in parallel
pool.map(
- lambda i: self._query_fragment(map, i, total_fragments, contig, final_mappings),
+ lambda i: self._query_fragment(map, i, total_fragments, mem, kind, stride, final_mappings),
range(fragment_count),
)
-
# record the number of fragments
total_fragments += fragment_count
total_length += slen
- #
- # printf("FINALL MAPPINGS: %i\n", final_mappings._vec.size())
-
# compute core genomic identity after successful mapping
with nogil:
computeCGI(