From a082f5b64a68d0dbd5ce0c32643ffb48ba744136 Mon Sep 17 00:00:00 2001 From: Constantin Pape <constantin.pape@iwr.uni-heidelberg.de> Date: Mon, 8 Jul 2019 14:33:56 +0200 Subject: [PATCH] Update prospr segmented regions --- ...-6dpf-1-whole-segmented-CrypticSegment.xml | 12 +++++----- .../prospr-6dpf-1-whole-segmented-Head.xml | 12 +++++----- .../prospr-6dpf-1-whole-segmented-PNS.xml | 12 +++++----- ...prospr-6dpf-1-whole-segmented-Pygidium.xml | 12 +++++----- ...pr-6dpf-1-whole-segmented-RestOfAnimal.xml | 12 +++++----- ...rospr-6dpf-1-whole-segmented-Stomodeum.xml | 12 +++++----- .../prospr-6dpf-1-whole-segmented-VNC.xml | 12 +++++----- make_initial_version.py | 22 +++++++++++++++++++ 8 files changed, 64 insertions(+), 42 deletions(-) diff --git a/data/rawdata/prospr-6dpf-1-whole-segmented-CrypticSegment.xml b/data/rawdata/prospr-6dpf-1-whole-segmented-CrypticSegment.xml index 4272f85..c756b3c 100644 --- a/data/rawdata/prospr-6dpf-1-whole-segmented-CrypticSegment.xml +++ b/data/rawdata/prospr-6dpf-1-whole-segmented-CrypticSegment.xml @@ -5,6 +5,12 @@ <hdf5 type="relative">prospr-6dpf-1-whole-segmented-CrypticSegment.h5</hdf5> </ImageLoader> <ViewSetups> + <Attributes name="channel"> + <Channel> + <id>1</id> + <name>1</name> + </Channel> + </Attributes> <ViewSetup> <id>0</id> <name>channel 1</name> @@ -17,12 +23,6 @@ <channel>1</channel> </attributes> </ViewSetup> - <Attributes name="channel"> - <Channel> - <id>1</id> - <name>1</name> - </Channel> - </Attributes> </ViewSetups> <Timepoints type="range"> <first>0</first> diff --git a/data/rawdata/prospr-6dpf-1-whole-segmented-Head.xml b/data/rawdata/prospr-6dpf-1-whole-segmented-Head.xml index 1d624ec..de6c251 100644 --- a/data/rawdata/prospr-6dpf-1-whole-segmented-Head.xml +++ b/data/rawdata/prospr-6dpf-1-whole-segmented-Head.xml @@ -5,6 +5,12 @@ <hdf5 type="relative">prospr-6dpf-1-whole-segmented-Head.h5</hdf5> </ImageLoader> <ViewSetups> + <Attributes name="channel"> + <Channel> + <id>1</id> + <name>1</name> + </Channel> + </Attributes> <ViewSetup> <id>0</id> <name>channel 1</name> @@ -17,12 +23,6 @@ <channel>1</channel> </attributes> </ViewSetup> - <Attributes name="channel"> - <Channel> - <id>1</id> - <name>1</name> - </Channel> - </Attributes> </ViewSetups> <Timepoints type="range"> <first>0</first> diff --git a/data/rawdata/prospr-6dpf-1-whole-segmented-PNS.xml b/data/rawdata/prospr-6dpf-1-whole-segmented-PNS.xml index 2e3f4c9..fd9e36f 100644 --- a/data/rawdata/prospr-6dpf-1-whole-segmented-PNS.xml +++ b/data/rawdata/prospr-6dpf-1-whole-segmented-PNS.xml @@ -5,6 +5,12 @@ <hdf5 type="relative">prospr-6dpf-1-whole-segmented-PNS.h5</hdf5> </ImageLoader> <ViewSetups> + <Attributes name="channel"> + <Channel> + <id>1</id> + <name>1</name> + </Channel> + </Attributes> <ViewSetup> <id>0</id> <name>channel 1</name> @@ -17,12 +23,6 @@ <channel>1</channel> </attributes> </ViewSetup> - <Attributes name="channel"> - <Channel> - <id>1</id> - <name>1</name> - </Channel> - </Attributes> </ViewSetups> <Timepoints type="range"> <first>0</first> diff --git a/data/rawdata/prospr-6dpf-1-whole-segmented-Pygidium.xml b/data/rawdata/prospr-6dpf-1-whole-segmented-Pygidium.xml index d2d0ccb..b9b4060 100644 --- a/data/rawdata/prospr-6dpf-1-whole-segmented-Pygidium.xml +++ b/data/rawdata/prospr-6dpf-1-whole-segmented-Pygidium.xml @@ -5,6 +5,12 @@ <hdf5 type="relative">prospr-6dpf-1-whole-segmented-Pygidium.h5</hdf5> </ImageLoader> <ViewSetups> + <Attributes name="channel"> + <Channel> + <id>1</id> + <name>1</name> + </Channel> + </Attributes> <ViewSetup> <id>0</id> <name>channel 1</name> @@ -17,12 +23,6 @@ <channel>1</channel> </attributes> </ViewSetup> - <Attributes name="channel"> - <Channel> - <id>1</id> - <name>1</name> - </Channel> - </Attributes> </ViewSetups> <Timepoints type="range"> <first>0</first> diff --git a/data/rawdata/prospr-6dpf-1-whole-segmented-RestOfAnimal.xml b/data/rawdata/prospr-6dpf-1-whole-segmented-RestOfAnimal.xml index ff71c06..d97dd9d 100644 --- a/data/rawdata/prospr-6dpf-1-whole-segmented-RestOfAnimal.xml +++ b/data/rawdata/prospr-6dpf-1-whole-segmented-RestOfAnimal.xml @@ -5,6 +5,12 @@ <hdf5 type="relative">prospr-6dpf-1-whole-segmented-RestOfAnimal.h5</hdf5> </ImageLoader> <ViewSetups> + <Attributes name="channel"> + <Channel> + <id>1</id> + <name>1</name> + </Channel> + </Attributes> <ViewSetup> <id>0</id> <name>channel 1</name> @@ -17,12 +23,6 @@ <channel>1</channel> </attributes> </ViewSetup> - <Attributes name="channel"> - <Channel> - <id>1</id> - <name>1</name> - </Channel> - </Attributes> </ViewSetups> <Timepoints type="range"> <first>0</first> diff --git a/data/rawdata/prospr-6dpf-1-whole-segmented-Stomodeum.xml b/data/rawdata/prospr-6dpf-1-whole-segmented-Stomodeum.xml index 9439922..7f409ff 100644 --- a/data/rawdata/prospr-6dpf-1-whole-segmented-Stomodeum.xml +++ b/data/rawdata/prospr-6dpf-1-whole-segmented-Stomodeum.xml @@ -5,6 +5,12 @@ <hdf5 type="relative">prospr-6dpf-1-whole-segmented-Stomodeum.h5</hdf5> </ImageLoader> <ViewSetups> + <Attributes name="channel"> + <Channel> + <id>1</id> + <name>1</name> + </Channel> + </Attributes> <ViewSetup> <id>0</id> <name>channel 1</name> @@ -17,12 +23,6 @@ <channel>1</channel> </attributes> </ViewSetup> - <Attributes name="channel"> - <Channel> - <id>1</id> - <name>1</name> - </Channel> - </Attributes> </ViewSetups> <Timepoints type="range"> <first>0</first> diff --git a/data/rawdata/prospr-6dpf-1-whole-segmented-VNC.xml b/data/rawdata/prospr-6dpf-1-whole-segmented-VNC.xml index 5072ebd..77ea228 100644 --- a/data/rawdata/prospr-6dpf-1-whole-segmented-VNC.xml +++ b/data/rawdata/prospr-6dpf-1-whole-segmented-VNC.xml @@ -5,6 +5,12 @@ <hdf5 type="relative">prospr-6dpf-1-whole-segmented-VNC.h5</hdf5> </ImageLoader> <ViewSetups> + <Attributes name="channel"> + <Channel> + <id>1</id> + <name>1</name> + </Channel> + </Attributes> <ViewSetup> <id>0</id> <name>channel 1</name> @@ -17,12 +23,6 @@ <channel>1</channel> </attributes> </ViewSetup> - <Attributes name="channel"> - <Channel> - <id>1</id> - <name>1</name> - </Channel> - </Attributes> </ViewSetups> <Timepoints type="range"> <first>0</first> diff --git a/make_initial_version.py b/make_initial_version.py index aa993ae..f8528b2 100755 --- a/make_initial_version.py +++ b/make_initial_version.py @@ -2,11 +2,14 @@ import os from shutil import copyfile +from glob import glob +import h5py from scripts.files import make_folder_structure from scripts.export import export_segmentation from scripts.files import make_bdv_server_file, copy_image_data, copy_misc_data from scripts.attributes import make_nucleus_tables, make_cell_tables +from pybdv.converter import make_bdv def make_sbem_segmentations(old_folder, folder): @@ -51,6 +54,23 @@ def make_sbem_tables(folder): copyfile(tissue_table_in, tissue_table_out) +def make_prospr_region_segmentations(): + in_prefix = '/g/arendt/EM_6dpf_segmentation/EM-Prospr/BodyPart_*.h5' + out_prefix = './data/rawdata/prospr-6dpf-1-whole-segmented-' + files = glob(in_prefix) + for p in files: + name = p.split('_')[-1][:-3] + o = out_prefix + name + '.h5' + print(p, "to", o) + with h5py.File(p) as f: + key = 't00000/s00/0/cells' + data = f[key][:] + data[data > 0] = 0 + data[data < 0] = 255 + make_bdv(data, o, 3 * [[2, 2, 2]], + unit='micrometer', resolution=[0.5, 0.5, 0.5]) + + def make_initial_version(): src_folder = 'data/rawdata' @@ -76,5 +96,7 @@ def make_initial_version(): os.path.join(folder, 'misc', 'bdvserver.txt')) + if __name__ == '__main__': + # make_prospr_region_segmentations() make_initial_version() -- GitLab