diff --git a/README.md b/README.md
index 65ea51ad7f81e17f088654e808bc2891b6cb4e33..89482c16fc00a0bb6053ac6925e5abd7371ce9fb 100755
--- a/README.md
+++ b/README.md
@@ -205,7 +205,7 @@ infrastructure. Here is an example command:
 The provided environment `conda-environment.yml` is the result of running:
 
   ```
-  conda create -y -n strandseqnation snakemake=5.2 bcftools bioconductor-biobase bioconductor-biocgenerics bioconductor-biocinstaller bioconductor-biocparallel bioconductor-biostrings bioconductor-bsgenome bioconductor-bsgenome.hsapiens.ucsc.hg38 bioconductor-delayedarray bioconductor-fastseg bioconductor-genomeinfodb bioconductor-genomeinfodbdata bioconductor-genomicalignments bioconductor-genomicranges bioconductor-iranges bioconductor-rsamtools bioconductor-rtracklayer bioconductor-s4vectors bioconductor-summarizedexperiment bioconductor-xvector bioconductor-zlibbioc filechunkio ftputil htslib pysftp r-data.table r-peer samtools urllib3 aioeasywebdav aiohttp appdirs asn1crypto async-timeout boost boost-cpp bzip2 ca-certificates cairo certifi cffi chardet cmake configargparse cryptography curl docutils dropbox expat fontconfig freetype gettext glib graphite2 gsl harfbuzz icu idna jpeg krb5 libffi libiconv libpng libssh2 libtiff libuv libxml2 multidict ncurses openssl pandas pango paramiko pcre pip pixman psutil pyasn1 pycparser pynacl python python-dateutil pytz pyyaml r-assertthat r-base r-bh r-bitops r-colorspace r-cowplot r-curl r-devtools r-dichromat r-digest r-futile.logger r-futile.options r-ggplot2 r-git2r r-gridextra r-gtable r-hexbin r-httr r-jsonlite r-labeling r-lambda.r r-lattice r-lazyeval r-magrittr r-mass r-matrix r-matrixstats r-memoise r-mime r-munsell r-openssl r-plyr r-r6 r-rcolorbrewer r-rcpp r-rcurl r-reshape2 r-rlang r-rstudioapi r-scales r-snow r-stringi r-stringr r-tibble r-viridislite r-whisker r-withr r-xml ratelimiter readline requests rhash setuptools six sqlite tk wheel wrapt xz yaml yarl zlib bcrypt intel-openmp libgcc libgcc-ng libgfortran-ng libstdcxx-ng mkl numpy wget r-dplyr scipy whatshap freebayes r-mc2d
+  conda create -y -n strandseqnation snakemake=5.2 r-ggplot2=2.2 bcftools bioconductor-biobase bioconductor-biocgenerics bioconductor-biocinstaller bioconductor-biocparallel bioconductor-biostrings bioconductor-bsgenome bioconductor-bsgenome.hsapiens.ucsc.hg38 bioconductor-delayedarray bioconductor-fastseg bioconductor-genomeinfodb bioconductor-genomeinfodbdata bioconductor-genomicalignments bioconductor-genomicranges bioconductor-iranges bioconductor-rsamtools bioconductor-rtracklayer bioconductor-s4vectors bioconductor-summarizedexperiment bioconductor-xvector bioconductor-zlibbioc filechunkio ftputil htslib pysftp r-data.table r-peer samtools urllib3 aioeasywebdav aiohttp appdirs asn1crypto async-timeout boost boost-cpp bzip2 ca-certificates cairo certifi cffi chardet cmake configargparse cryptography curl docutils dropbox expat fontconfig freetype gettext glib graphite2 gsl harfbuzz icu idna jpeg krb5 libffi libiconv libpng libssh2 libtiff libuv libxml2 multidict ncurses openssl pandas pango paramiko pcre pip pixman psutil pyasn1 pycparser pynacl python python-dateutil pytz pyyaml r-assertthat r-base r-bh r-bitops r-colorspace r-cowplot r-curl r-devtools r-dichromat r-digest r-futile.logger r-futile.options r-git2r r-gridextra r-gtable r-hexbin r-httr r-jsonlite r-labeling r-lambda.r r-lattice r-lazyeval r-magrittr r-mass r-matrix r-matrixstats r-memoise r-mime r-munsell r-openssl r-plyr r-r6 r-rcolorbrewer r-rcpp r-rcurl r-reshape2 r-rlang r-rstudioapi r-scales r-snow r-stringi r-stringr r-tibble r-viridislite r-whisker r-withr r-xml ratelimiter readline requests rhash setuptools six sqlite tk wheel wrapt xz yaml yarl zlib bcrypt intel-openmp libgcc libgcc-ng libgfortran-ng libstdcxx-ng mkl numpy wget r-dplyr scipy whatshap freebayes r-mc2d
 
   conda env export > conda-environment.yml
   ```
diff --git a/conda-environment.yml b/conda-environment.yml
index 8b92b6f7e21ae722425d630b3227651f6e6ba07d..638f04efa19cb74f1816cef8379f587179649f42 100644
--- a/conda-environment.yml
+++ b/conda-environment.yml
@@ -150,7 +150,7 @@ dependencies:
   - r-dplyr=0.7.6=r341h9d2a408_0
   - r-futile.logger=1.4.3=r3.4.1_0
   - r-futile.options=1.0.0=r3.4.1_0
-  - r-ggplot2=3.0.0=r341h6115d3f_0
+  - r-ggplot2=2.2.1=r3.4.1_0
   - r-git2r=0.21.0=r341h0c37787_0
   - r-glue=1.2.0=r3.4.1_0
   - r-gridextra=2.3=r3.4.1_0
@@ -168,11 +168,9 @@ dependencies:
   - r-matrixstats=0.53.1=r341_0
   - r-mc2d=0.1_18=r3.4.1_0
   - r-memoise=1.1.0=r3.4.1_0
-  - r-mgcv=1.8_24=r341_0
   - r-mime=0.5=r3.4.1_0
   - r-munsell=0.5.0=r341_0
   - r-mvtnorm=1.0_6=r3.4.1_0
-  - r-nlme=3.1_131=r3.4.1_0
   - r-openssl=1.0.1=r3.4.1_0
   - r-pillar=1.2.2=r341h6115d3f_1
   - r-pkgconfig=2.0.1=r3.4.1_0