diff --git a/Snakefile b/Snakefile index e85e334ab63a262f5327f0a0ef5a90b1d859d6ac..5b3807839b4adb7cd1d14c12b1d533623c6c82c1 100644 --- a/Snakefile +++ b/Snakefile @@ -349,6 +349,20 @@ rule generate_halo_json: "(./utils/counts_to_json.py {input.counts} | gzip > {output.json}) 2> {log}" + +rule plot_clustering: + input: + sv_calls = "sv_calls/{sample}/{windows}.{bpdens}/{method}.txt", + binbed = "utils/bin_200kb_all.bed", + output: + position = "sv_calls/{sample}/{windows}.{bpdens,selected_j[0-9\\.]+_s[0-9\\.]+}/plots/sv_clustering/{method}-position.pdf", + chromosome = "sv_calls/{sample}/{windows}.{bpdens,selected_j[0-9\\.]+_s[0-9\\.]+}/plots/sv_clustering/{method}-chromosome.pdf", + log: + "log/plot_clustering/{sample}/{windows}.{bpdens}.{method}.log" + script: + "utils/plot-clustering.snakemake.R" + + ################################################################################ # Read counting # ################################################################################ diff 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56400000 bin_200kb_15425 +chrY 56400000 56600000 bin_200kb_15426 +chrY 56600000 56800000 bin_200kb_15427 +chrY 56800000 57000000 bin_200kb_15428 +chrY 57000000 57200000 bin_200kb_15429 diff --git a/utils/plot-clustering.R b/utils/plot-clustering.R index 89b03cf9ca8399bb9595c12187d282f8da44491b..c055b2bdeb269abbe8db3a36640cf238882e6dab 100644 --- a/utils/plot-clustering.R +++ b/utils/plot-clustering.R @@ -1,222 +1,210 @@ -setwd("/Users/jeong/Documents/Strand_Seq/TALL_analysis/sv_calls_new_1008") ##This folder need to be changed ( /path/to/sv_calls ) -genome_bins<-read.table("/Users/jeong/Documents/Strand_Seq/TALL_analysis/sv_calls/bin_200kb_all.bed", sep = '\t', header=F, comment.char = "") ##This folder need to be changed ( /path/to/genome bins bed file ) -list_directory <- dir("./", full.names=TRUE) - -for (m in 1:(length(list_directory))){ -files <- list.files(paste0(list_directory[m], '/100000_fixed_norm.selected_j0.1_s0.5'), pattern=".txt$", full.names=TRUE) -plotname<-paste0("Mosaic_plot_", strsplit(list_directory[m], './/')[[1]][2], "_position.pdf") -pdf(plotname, width = 11, height = 10 ) - -for (k in 1:length(files)){ - -filename<-strsplit(files[k], './/')[[1]][2] -data1<-read.table(files[k], sep = '\t', header=T, comment.char = "") - - -data1$color <- 0 -ash12rainbow <- c("#77AADD", "#4477AA", "#114477", "#CC99BB", "#AA4488", "#771155", "#DDDD77", "#AAAA44", "#777711", "#DDAA77", "#AA7744", "#774411") -sv_call_name <- c("del_h1", "del_h2", "del_hom", "dup_h1", "dup_h2", "dup_hom", "inv_h1", "inv_h2", "inv_hom", "idup_h1", "idup_h2", "complex") -for (j in 1:length(sv_call_name)){ -tmp <- which(data1[,9]==sv_call_name[j]) -data1[tmp,15] <- j -} - - - -data1_pos <- data1[,1:3] -data1_pos_uniq <- unique(data1_pos) -data1_pos_uniq_sort <- data1_pos_uniq[data1_pos_uniq$chrom=="chr1",] -chrom <- c("chr1", "chr2", "chr3", "chr4", "chr5", "chr6", "chr7", "chr8", "chr9", "chr10", "chr11", "chr12", "chr13", "chr14", "chr15", "chr16", "chr17", "chr18", "chr19", "chr20", "chr21", "chr22", "chrX") - -for (i in 2:length(chrom)){ -data1_pos_uniq_sort<-rbind(data1_pos_uniq_sort, data1_pos_uniq[data1_pos_uniq$chrom==chrom[i],]) -} -data1_pos_uniq_sort$posind <- c(1:nrow(data1_pos_uniq_sort)) - -data1_cell <- data1[,5] -data1_cell_uniq <- unique(data1_cell) -data1_cell_uniq_sort <- as.matrix(sort(data1_cell_uniq)) - -result <- matrix(0, nrow(data1_cell_uniq_sort), nrow(data1_pos_uniq)) -result_sv <- matrix(0, nrow(data1_cell_uniq_sort), nrow(data1_pos_uniq)) - -for (i in 1:nrow(data1)){ -pos_ind <- which(data1_pos_uniq_sort[,1]==data1[i,1] & data1_pos_uniq_sort[,2]==data1[i,2] & data1_pos_uniq_sort[,3]==data1[i,3]) -cell_ind <- which(data1_cell_uniq_sort[,1]==data1[i,5]) -result[cell_ind, pos_ind] <- data1[i,13] -result_sv[cell_ind, pos_ind] <- data1[i,15] -} -rownames(result) <- data1_cell_uniq_sort -colnames(result) <- data1_pos_uniq_sort$posind -result[result==Inf] <- max(result[result!=Inf]) -rownames(result_sv) <- data1_cell_uniq_sort -colnames(result_sv) <- data1_pos_uniq_sort$posind - -##Add chromosome color code to heatmap -data1_pos_uniq_sort$color <- "magenta" -data1_pos_uniq_sort[data1_pos_uniq_sort$chrom=="chr2",5] <- "purple" -data1_pos_uniq_sort[data1_pos_uniq_sort$chr=="chr4",5] <- "purple" -data1_pos_uniq_sort[data1_pos_uniq_sort$chr=="chr6",5] <- "purple" -data1_pos_uniq_sort[data1_pos_uniq_sort$chr=="chr8",5] <- "purple" -data1_pos_uniq_sort[data1_pos_uniq_sort$chr=="chr10",5] <- "purple" -data1_pos_uniq_sort[data1_pos_uniq_sort$chr=="chr12",5] <- "purple" -data1_pos_uniq_sort[data1_pos_uniq_sort$chr=="chr14",5] <- "purple" -data1_pos_uniq_sort[data1_pos_uniq_sort$chr=="chr16",5] <- "purple" -data1_pos_uniq_sort[data1_pos_uniq_sort$chr=="chr18",5] <- "purple" -data1_pos_uniq_sort[data1_pos_uniq_sort$chr=="chr20",5] <- "purple" -data1_pos_uniq_sort[data1_pos_uniq_sort$chr=="chr22",5] <- "purple" -data1_pos_uniq_sort[data1_pos_uniq_sort$chr=="chrY",5] <- "purple" - - -library(pheatmap) -library(gplots) -library(ComplexHeatmap) -breaksList = seq(4, 30, by = 0.1) -breaksList=append(breaksList, max(result)) -breaksList=append(breaksList, -1, 0) -mycol <- colorpanel(n=length(breaksList)-1,low="lightgoldenrodyellow", mid = "darkorange", high="firebrick2") - -Var1 <- c("magenta", "purple") -names(Var1) <- c("magenta", "purple") -anno_colors <- list(Var1 = Var1) -col_annotation <- as.data.frame(data1_pos_uniq_sort$color) -rownames(col_annotation) <- colnames(result) -colnames(col_annotation) <- "Var1" - - -res<-pheatmap(result, border_color = NA, show_rownames=T, show_colnames=F, cluster_cols=F, cluster_rows=T, clustering_method="ward.D", scale="none", col=mycol, cex=0.7, main=filename, annotation_col = col_annotation, annotation_colors = anno_colors, breaks = breaksList, annotation_legend = FALSE) -#pheatmap(result_sv[res$tree_row$order,], border_color = NA, show_rownames=T, show_colnames=F, cluster_cols=F, cluster_rows=F, scale="none", cex=0.7, col=c("white", "#77AADD", "#4477AA", "#114477", "#CC99BB", "#AA4488", "#771155", "#DDDD77", "#AAAA44", "#777711", "#DDAA77", "#AA7744", "#774411"), main=filename, annotation_col = col_annotation, annotation_colors = anno_colors, annotation_legend = FALSE, legend=TRUE, legend_labels <- c("none", "del_h1", "del_h2", "del_hom", "dup_h1", "dup_h2", "dup_hom", "inv_h1", "inv_h2", "inv_hom", "idup_h1", "idup_h2", "complex")) - -chr_name <- matrix("chr", ncol(result), 1) -for (i in 1:ncol(result)){ -chr_name[i,1] <- paste0(data1_pos_uniq_sort[i,1], '_', data1_pos_uniq_sort[i,2], '_', data1_pos_uniq_sort[i,3]) -} -colnames(result_sv) <- chr_name -colors = structure(c("white", "#77AADD", "#4477AA", "#114477", "#CC99BB", "#AA4488", "#771155", "#DDDD77", "#AAAA44", "#777711", "#DDAA77", "#AA7744", "#774411"), names = c(0:12)) - -sv_list = c("none", "del_h1", "del_h2", "del_hom", "dup_h1", "dup_h2", "dup_hom", "inv_h1", "inv_h2", "inv_hom", "idup_h1", "idup_h2", "complex") -sv_tmp <- matrix(0, 13, 1) -for (i in 1:13){sv_tmp[i,1] <- sum(result_sv==(i-1)) } -sv_list_sub <- sv_list[sv_tmp>0] - -mat <- as.data.frame(result_sv[res$tree_row$order,]) -ha_column = HeatmapAnnotation(df = data.frame(type1 = data1_pos_uniq_sort$color), - col = list(type1 = c("magenta" = "magenta", "purple" = "purple"))) -#ht1 = Heatmap(mat, name = "", col=c("white", "#77AADD", "#4477AA", "#114477", "#CC99BB", "#AA4488", "#771155", "#DDDD77", "#AAAA44", "#777711", "#DDAA77", "#AA7744", "#774411"), heatmap_legend_param = list(labels = c("none", "del_h1", "del_h2", "del_hom", "dup_h1", "dup_h2", "dup_hom", "inv_h1", "inv_h2", "inv_hom", "idup_h1", "idup_h2", "complex")),cluster_rows = FALSE, cluster_columns = FALSE, column_title = filename, row_names_gp = gpar(fontsize = 5), column_names_gp = gpar(fontsize = 3), column_title_gp = gpar(fontsize = 7, fontface = "bold"), top_annotation = ha_column) -ht1 = Heatmap(mat, name = "", col=colors, heatmap_legend_param = list(labels = sv_list_sub),cluster_rows = FALSE, cluster_columns = FALSE, column_title = filename, row_names_gp = gpar(fontsize = 5), column_names_gp = gpar(fontsize = 1), column_title_gp = gpar(fontsize = 7, fontface = "bold"), top_annotation = ha_column) -draw(ht1, show_annotation_legend = FALSE) - - -} -dev.off() - - - -files <- list.files(paste0(list_directory[m], '/100000_fixed_norm.selected_j0.1_s0.5'), pattern=".txt$", full.names=TRUE) -plotname<-paste0("Mosaic_plot_", strsplit(list_directory[m], './/')[[1]][2], "_chromosome.pdf") -pdf(plotname, width = 11, height = 5 ) - -for (k in 1:length(files)){ - -filename<-strsplit(files[k], './/')[[1]][2] -data1<-read.table(files[k], sep = '\t', header=T, comment.char = "") - - -data1$color <- 0 -ash12rainbow <- c("#77AADD", "#4477AA", "#114477", "#CC99BB", "#AA4488", "#771155", "#DDDD77", "#AAAA44", "#777711", "#DDAA77", "#AA7744", "#774411") -sv_call_name <- c("del_h1", "del_h2", "del_hom", "dup_h1", "dup_h2", "dup_hom", "inv_h1", "inv_h2", "inv_hom", "idup_h1", "idup_h2", "complex") -for (j in 1:length(sv_call_name)){ -tmp <- which(data1[,9]==sv_call_name[j]) -data1[tmp,15] <- j -} -colors = structure(c("white", "#77AADD", "#4477AA", "#114477", "#CC99BB", "#AA4488", "#771155", "#DDDD77", "#AAAA44", "#777711", "#DDAA77", "#AA7744", "#774411"), names = c(0:12)) - - -data1_pos <- data1[,1:3] -data1_pos_uniq <- unique(data1_pos) -data1_pos_uniq_sort <- data1_pos_uniq[data1_pos_uniq$chrom=="chr1",] -chrom <- c("chr1", "chr2", "chr3", "chr4", "chr5", "chr6", "chr7", "chr8", "chr9", "chr10", "chr11", "chr12", "chr13", "chr14", "chr15", "chr16", "chr17", "chr18", "chr19", "chr20", "chr21", "chr22", "chrX") - -for (i in 2:length(chrom)){ -data1_pos_uniq_sort<-rbind(data1_pos_uniq_sort, data1_pos_uniq[data1_pos_uniq$chrom==chrom[i],]) -} -data1_pos_uniq_sort$posind <- c(1:nrow(data1_pos_uniq_sort)) - -data1_cell <- data1[,5] -data1_cell_uniq <- unique(data1_cell) -data1_cell_uniq_sort <- as.matrix(sort(data1_cell_uniq)) - -result <- matrix(0, nrow(data1_cell_uniq_sort), nrow(data1_pos_uniq)) -result_sv <- matrix(0, nrow(data1_cell_uniq_sort), nrow(data1_pos_uniq)) - -for (i in 1:nrow(data1)){ -pos_ind <- which(data1_pos_uniq_sort[,1]==data1[i,1] & data1_pos_uniq_sort[,2]==data1[i,2] & data1_pos_uniq_sort[,3]==data1[i,3]) -cell_ind <- which(data1_cell_uniq_sort[,1]==data1[i,5]) -result[cell_ind, pos_ind] <- data1[i,13] -result_sv[cell_ind, pos_ind] <- data1[i,15] -} -rownames(result) <- data1_cell_uniq_sort -colnames(result) <- data1_pos_uniq_sort$posind -result[result==Inf] <- max(result[result!=Inf]) -rownames(result_sv) <- data1_cell_uniq_sort -colnames(result_sv) <- data1_pos_uniq_sort$posind - +plot.clustering <- function(inputfile, bin.bed.filename, position.outputfile, chromosome.outputfile) { + +# setwd("/Users/jeong/Documents/Strand_Seq/TALL_analysis/sv_calls_new_1008") ##This folder need to be changed ( /path/to/sv_calls ) + genome_bins <- read.table(bin.bed.filename, sep = '\t', header=F, comment.char = "") + list_directory <- dir("./", full.names=TRUE) + +# files <- list.files(paste0(list_directory[m], '/100000_fixed_norm.selected_j0.1_s0.5'), pattern=".txt$", full.names=TRUE) +# plotname<-paste0("Mosaic_plot_", strsplit(list_directory[m], './/')[[1]][2], "_position.pdf") + pdf(position.outputfile, width = 11, height = 10 ) + + +# filename<-strsplit(files[k], './/')[[1]][2] + data1<-read.table(inputfile, sep = '\t', header=T, comment.char = "") + + + data1$color <- 0 + ash12rainbow <- c("#77AADD", "#4477AA", "#114477", "#CC99BB", "#AA4488", "#771155", "#DDDD77", "#AAAA44", "#777711", "#DDAA77", "#AA7744", "#774411") + sv_call_name <- c("del_h1", "del_h2", "del_hom", "dup_h1", "dup_h2", "dup_hom", "inv_h1", "inv_h2", "inv_hom", "idup_h1", "idup_h2", "complex") + for (j in 1:length(sv_call_name)){ + tmp <- which(data1[,9]==sv_call_name[j]) + data1[tmp,15] <- j + } + + + + data1_pos <- data1[,1:3] + data1_pos_uniq <- unique(data1_pos) + data1_pos_uniq_sort <- data1_pos_uniq[data1_pos_uniq$chrom=="chr1",] + chrom <- c("chr1", "chr2", "chr3", "chr4", "chr5", "chr6", "chr7", "chr8", "chr9", "chr10", "chr11", "chr12", "chr13", "chr14", "chr15", "chr16", "chr17", "chr18", "chr19", "chr20", "chr21", "chr22", "chrX") + + for (i in 2:length(chrom)){ + data1_pos_uniq_sort<-rbind(data1_pos_uniq_sort, data1_pos_uniq[data1_pos_uniq$chrom==chrom[i],]) + } + data1_pos_uniq_sort$posind <- c(1:nrow(data1_pos_uniq_sort)) + + data1_cell <- data1[,5] + data1_cell_uniq <- unique(data1_cell) + data1_cell_uniq_sort <- as.matrix(sort(data1_cell_uniq)) + + result <- matrix(0, nrow(data1_cell_uniq_sort), nrow(data1_pos_uniq)) + result_sv <- matrix(0, nrow(data1_cell_uniq_sort), nrow(data1_pos_uniq)) + + for (i in 1:nrow(data1)){ + pos_ind <- which(data1_pos_uniq_sort[,1]==data1[i,1] & data1_pos_uniq_sort[,2]==data1[i,2] & data1_pos_uniq_sort[,3]==data1[i,3]) + cell_ind <- which(data1_cell_uniq_sort[,1]==data1[i,5]) + result[cell_ind, pos_ind] <- data1[i,13] + result_sv[cell_ind, pos_ind] <- data1[i,15] + } + rownames(result) <- data1_cell_uniq_sort + colnames(result) <- data1_pos_uniq_sort$posind + result[result==Inf] <- max(result[result!=Inf]) + rownames(result_sv) <- data1_cell_uniq_sort + colnames(result_sv) <- data1_pos_uniq_sort$posind + + ##Add chromosome color code to heatmap + data1_pos_uniq_sort$color <- "magenta" + data1_pos_uniq_sort[data1_pos_uniq_sort$chrom=="chr2",5] <- "purple" + data1_pos_uniq_sort[data1_pos_uniq_sort$chr=="chr4",5] <- "purple" + data1_pos_uniq_sort[data1_pos_uniq_sort$chr=="chr6",5] <- "purple" + data1_pos_uniq_sort[data1_pos_uniq_sort$chr=="chr8",5] <- "purple" + data1_pos_uniq_sort[data1_pos_uniq_sort$chr=="chr10",5] <- "purple" + data1_pos_uniq_sort[data1_pos_uniq_sort$chr=="chr12",5] <- "purple" + data1_pos_uniq_sort[data1_pos_uniq_sort$chr=="chr14",5] <- "purple" + data1_pos_uniq_sort[data1_pos_uniq_sort$chr=="chr16",5] <- "purple" + data1_pos_uniq_sort[data1_pos_uniq_sort$chr=="chr18",5] <- "purple" + data1_pos_uniq_sort[data1_pos_uniq_sort$chr=="chr20",5] <- "purple" + data1_pos_uniq_sort[data1_pos_uniq_sort$chr=="chr22",5] <- "purple" + data1_pos_uniq_sort[data1_pos_uniq_sort$chr=="chrY",5] <- "purple" + + + library(pheatmap) + library(gplots) + library(ComplexHeatmap) + breaksList = seq(4, 30, by = 0.1) + breaksList=append(breaksList, max(result)) + breaksList=append(breaksList, -1, 0) + mycol <- colorpanel(n=length(breaksList)-1,low="lightgoldenrodyellow", mid = "darkorange", high="firebrick2") + + Var1 <- c("magenta", "purple") + names(Var1) <- c("magenta", "purple") + anno_colors <- list(Var1 = Var1) + col_annotation <- as.data.frame(data1_pos_uniq_sort$color) + rownames(col_annotation) <- colnames(result) + colnames(col_annotation) <- "Var1" + + + res<-pheatmap(result, border_color = NA, show_rownames=T, show_colnames=F, cluster_cols=F, cluster_rows=T, clustering_method="ward.D", scale="none", col=mycol, cex=0.7, main=inputfile, annotation_col = col_annotation, annotation_colors = anno_colors, breaks = breaksList, annotation_legend = FALSE) + #pheatmap(result_sv[res$tree_row$order,], border_color = NA, show_rownames=T, show_colnames=F, cluster_cols=F, cluster_rows=F, scale="none", cex=0.7, col=c("white", "#77AADD", "#4477AA", "#114477", "#CC99BB", "#AA4488", "#771155", "#DDDD77", "#AAAA44", "#777711", "#DDAA77", "#AA7744", "#774411"), main=filename, annotation_col = col_annotation, annotation_colors = anno_colors, annotation_legend = FALSE, legend=TRUE, legend_labels <- c("none", "del_h1", "del_h2", "del_hom", "dup_h1", "dup_h2", "dup_hom", "inv_h1", "inv_h2", "inv_hom", "idup_h1", "idup_h2", "complex")) + + chr_name <- matrix("chr", ncol(result), 1) + for (i in 1:ncol(result)){ + chr_name[i,1] <- paste0(data1_pos_uniq_sort[i,1], '_', data1_pos_uniq_sort[i,2], '_', data1_pos_uniq_sort[i,3]) + } + colnames(result_sv) <- chr_name + colors = structure(c("white", "#77AADD", "#4477AA", "#114477", "#CC99BB", "#AA4488", "#771155", "#DDDD77", "#AAAA44", "#777711", "#DDAA77", "#AA7744", "#774411"), names = c(0:12)) + + sv_list = c("none", "del_h1", "del_h2", "del_hom", "dup_h1", "dup_h2", "dup_hom", "inv_h1", "inv_h2", "inv_hom", "idup_h1", "idup_h2", "complex") + sv_tmp <- matrix(0, 13, 1) + for (i in 1:13){sv_tmp[i,1] <- sum(result_sv==(i-1)) } + sv_list_sub <- sv_list[sv_tmp>0] + + mat <- as.data.frame(result_sv[res$tree_row$order,]) + ha_column = HeatmapAnnotation(df = data.frame(type1 = data1_pos_uniq_sort$color), + col = list(type1 = c("magenta" = "magenta", "purple" = "purple"))) + #ht1 = Heatmap(mat, name = "", col=c("white", "#77AADD", "#4477AA", "#114477", "#CC99BB", "#AA4488", "#771155", "#DDDD77", "#AAAA44", "#777711", "#DDAA77", "#AA7744", "#774411"), heatmap_legend_param = list(labels = c("none", "del_h1", "del_h2", "del_hom", "dup_h1", "dup_h2", "dup_hom", "inv_h1", "inv_h2", "inv_hom", "idup_h1", "idup_h2", "complex")),cluster_rows = FALSE, cluster_columns = FALSE, column_title = filename, row_names_gp = gpar(fontsize = 5), column_names_gp = gpar(fontsize = 3), column_title_gp = gpar(fontsize = 7, fontface = "bold"), top_annotation = ha_column) + ht1 = Heatmap(mat, name = "", col=colors, heatmap_legend_param = list(labels = sv_list_sub),cluster_rows = FALSE, cluster_columns = FALSE, column_title = inputfile, row_names_gp = gpar(fontsize = 5), column_names_gp = gpar(fontsize = 1), column_title_gp = gpar(fontsize = 7, fontface = "bold"), top_annotation = ha_column) + draw(ht1, show_annotation_legend = FALSE) + + dev.off() + + pdf(chromosome.outputfile, width = 11, height = 5 ) + + + data1<-read.table(inputfile, sep = '\t', header=T, comment.char = "") + + data1$color <- 0 + ash12rainbow <- c("#77AADD", "#4477AA", "#114477", "#CC99BB", "#AA4488", "#771155", "#DDDD77", "#AAAA44", "#777711", "#DDAA77", "#AA7744", "#774411") + sv_call_name <- c("del_h1", "del_h2", "del_hom", "dup_h1", "dup_h2", "dup_hom", "inv_h1", "inv_h2", "inv_hom", "idup_h1", "idup_h2", "complex") + for (j in 1:length(sv_call_name)){ + tmp <- which(data1[,9]==sv_call_name[j]) + data1[tmp,15] <- j + } + colors = structure(c("white", "#77AADD", "#4477AA", "#114477", "#CC99BB", "#AA4488", "#771155", "#DDDD77", "#AAAA44", "#777711", "#DDAA77", "#AA7744", "#774411"), names = c(0:12)) + + + data1_pos <- data1[,1:3] + data1_pos_uniq <- unique(data1_pos) + data1_pos_uniq_sort <- data1_pos_uniq[data1_pos_uniq$chrom=="chr1",] + chrom <- c("chr1", "chr2", "chr3", "chr4", "chr5", "chr6", "chr7", "chr8", "chr9", "chr10", "chr11", "chr12", "chr13", "chr14", "chr15", "chr16", "chr17", "chr18", "chr19", "chr20", "chr21", "chr22", "chrX") + + for (i in 2:length(chrom)){ + data1_pos_uniq_sort<-rbind(data1_pos_uniq_sort, data1_pos_uniq[data1_pos_uniq$chrom==chrom[i],]) + } + data1_pos_uniq_sort$posind <- c(1:nrow(data1_pos_uniq_sort)) + + data1_cell <- data1[,5] + data1_cell_uniq <- unique(data1_cell) + data1_cell_uniq_sort <- as.matrix(sort(data1_cell_uniq)) + + result <- matrix(0, nrow(data1_cell_uniq_sort), nrow(data1_pos_uniq)) + result_sv <- matrix(0, nrow(data1_cell_uniq_sort), nrow(data1_pos_uniq)) + + for (i in 1:nrow(data1)){ + pos_ind <- which(data1_pos_uniq_sort[,1]==data1[i,1] & data1_pos_uniq_sort[,2]==data1[i,2] & data1_pos_uniq_sort[,3]==data1[i,3]) + cell_ind <- which(data1_cell_uniq_sort[,1]==data1[i,5]) + result[cell_ind, pos_ind] <- data1[i,13] + result_sv[cell_ind, pos_ind] <- data1[i,15] + } + rownames(result) <- data1_cell_uniq_sort + colnames(result) <- data1_pos_uniq_sort$posind + result[result==Inf] <- max(result[result!=Inf]) + rownames(result_sv) <- data1_cell_uniq_sort + colnames(result_sv) <- data1_pos_uniq_sort$posind + + + + library(pheatmap) + library(gplots) + breaksList = seq(4, 30, by = 0.1) + breaksList=append(breaksList, max(result)) + breaksList=append(breaksList, -1, 0) + mycol <- colorpanel(n=length(breaksList)-1,low="lightgoldenrodyellow", mid = "darkorange", high="firebrick2") + + res<-pheatmap(result, border_color = NA, show_rownames=T, show_colnames=F, cluster_cols=F, cluster_rows=T, clustering_method="ward.D", scale="none", col=mycol, cex=0.5, main=inputfile) + + + + ##Assign sv calls to the genome-wide bins (200kb) + par("mar") + par(mar=c(0.5,0.5,0.5,0.5)) + + genome_bins_sort <- genome_bins[genome_bins[,1]==chrom[1],] + for (i in 2:length(chrom)){ + genome_bins_sort <- rbind(genome_bins_sort, genome_bins[genome_bins[,1]==chrom[i],]) + } + + result_sv_bins <- matrix(0, nrow(genome_bins_sort), nrow(result_sv)) + for (i in 1:ncol(result_sv)){ + num_bins1 <- which(genome_bins_sort[,1]==as.character(data1_pos_uniq_sort[i,1]) & genome_bins_sort[,2]<=data1_pos_uniq_sort[i,2] & genome_bins_sort[,3]>data1_pos_uniq_sort[i,2]) + num_bins2 <- which(genome_bins_sort[,1]==as.character(data1_pos_uniq_sort[i,1]) & genome_bins_sort[,2]<=data1_pos_uniq_sort[i,3] & genome_bins_sort[,3]>data1_pos_uniq_sort[i,3]) + if (sum(genome_bins_sort[,1]==as.character(data1_pos_uniq_sort[i,1]) & genome_bins_sort[,2]<=data1_pos_uniq_sort[i,3] & genome_bins_sort[,3]>data1_pos_uniq_sort[i,3])==0){ + num_bins2 <- max(which(genome_bins_sort[,1]==as.character(data1_pos_uniq_sort[i,1]))) + } + + num_bins <- c(num_bins1:num_bins2) + for (j in 1:length(num_bins)){result_sv_bins[num_bins[j],] <- t(as.matrix(result_sv[,i]))} + cat(paste0(i, ' ')) + } + + widths <- matrix(0, length(chrom), 1) + for (i in 1:length(chrom)){widths[i] <- sum(genome_bins_sort[,1]==chrom[i])/100} + + colnames(genome_bins_sort)<-colnames(data1_pos_uniq_sort) + gos2 <- genome_bins_sort + mat2 <- result_sv_bins[,res$tree_row$order] + mat2 <- mat2[,ncol(mat2):1] + dnull <- matrix(0, nrow(mat2), ncol(mat2)) + #par(mfrow=c(1, 23)) + l <- layout(matrix(seq(1,23),1,23,byrow=TRUE), widths=widths) + for (i in 1:length(chrom)){ + d = mat2[gos2$chrom==chrom[i],] + #if (sum(d)==1){image(seq_len(1), seq_len(length(d)), as.matrix(t(d)), zlim=c(0, 12), col=colors, xlab="", ylab="", axes=FALSE, main=chrom[i], cex.main=0.8);box() } + if (sum(d)==0){image(seq_len(nrow(dnull)), seq_len(ncol(dnull)), dnull, zlim=c(0, 12), col="white", xlab="", ylab="", axes=FALSE, main=chrom[i], cex.main=0.8);box() } + if (sum(d)>1){image(seq_len(nrow(d)), seq_len(ncol(d)), d, zlim=c(0, 12), col=colors, xlab="", ylab="", axes=FALSE, main=chrom[i], cex.main=0.8);box() } + } + + dev.off() -library(pheatmap) -library(gplots) -breaksList = seq(4, 30, by = 0.1) -breaksList=append(breaksList, max(result)) -breaksList=append(breaksList, -1, 0) -mycol <- colorpanel(n=length(breaksList)-1,low="lightgoldenrodyellow", mid = "darkorange", high="firebrick2") - -res<-pheatmap(result, border_color = NA, show_rownames=T, show_colnames=F, cluster_cols=F, cluster_rows=T, clustering_method="ward.D", scale="none", col=mycol, cex=0.5, main=filename) - - - -##Assign sv calls to the genome-wide bins (200kb) -par("mar") -par(mar=c(0.5,0.5,0.5,0.5)) - -genome_bins_sort <- genome_bins[genome_bins[,1]==chrom[1],] -for (i in 2:length(chrom)){ -genome_bins_sort <- rbind(genome_bins_sort, genome_bins[genome_bins[,1]==chrom[i],]) -} - -result_sv_bins <- matrix(0, nrow(genome_bins_sort), nrow(result_sv)) -for (i in 1:ncol(result_sv)){ -num_bins1 <- which(genome_bins_sort[,1]==as.character(data1_pos_uniq_sort[i,1]) & genome_bins_sort[,2]<=data1_pos_uniq_sort[i,2] & genome_bins_sort[,3]>data1_pos_uniq_sort[i,2]) -num_bins2 <- which(genome_bins_sort[,1]==as.character(data1_pos_uniq_sort[i,1]) & genome_bins_sort[,2]<=data1_pos_uniq_sort[i,3] & genome_bins_sort[,3]>data1_pos_uniq_sort[i,3]) -if (sum(genome_bins_sort[,1]==as.character(data1_pos_uniq_sort[i,1]) & genome_bins_sort[,2]<=data1_pos_uniq_sort[i,3] & genome_bins_sort[,3]>data1_pos_uniq_sort[i,3])==0){ -num_bins2 <- max(which(genome_bins_sort[,1]==as.character(data1_pos_uniq_sort[i,1]))) -} - -num_bins <- c(num_bins1:num_bins2) -for (j in 1:length(num_bins)){result_sv_bins[num_bins[j],] <- t(as.matrix(result_sv[,i]))} -cat(paste0(i, ' ')) -} - -widths <- matrix(0, length(chrom), 1) -for (i in 1:length(chrom)){widths[i] <- sum(genome_bins_sort[,1]==chrom[i])/100} - -colnames(genome_bins_sort)<-colnames(data1_pos_uniq_sort) -gos2 <- genome_bins_sort -mat2 <- result_sv_bins[,res$tree_row$order] -mat2 <- mat2[,ncol(mat2):1] -dnull <- matrix(0, nrow(mat2), ncol(mat2)) -#par(mfrow=c(1, 23)) -l <- layout(matrix(seq(1,23),1,23,byrow=TRUE), widths=widths) -for (i in 1:length(chrom)){ -d = mat2[gos2$chrom==chrom[i],] -#if (sum(d)==1){image(seq_len(1), seq_len(length(d)), as.matrix(t(d)), zlim=c(0, 12), col=colors, xlab="", ylab="", axes=FALSE, main=chrom[i], cex.main=0.8);box() } -if (sum(d)==0){image(seq_len(nrow(dnull)), seq_len(ncol(dnull)), dnull, zlim=c(0, 12), col="white", xlab="", ylab="", axes=FALSE, main=chrom[i], cex.main=0.8);box() } -if (sum(d)>1){image(seq_len(nrow(d)), seq_len(ncol(d)), d, zlim=c(0, 12), col=colors, xlab="", ylab="", axes=FALSE, main=chrom[i], cex.main=0.8);box() } -} - - -} -dev.off() - - - -} +} \ No newline at end of file diff --git a/utils/plot-clustering.snakemake.R b/utils/plot-clustering.snakemake.R new file mode 100644 index 0000000000000000000000000000000000000000..badf8bce07dadb592a0803b5b065591e70b39f4d --- /dev/null +++ b/utils/plot-clustering.snakemake.R @@ -0,0 +1,6 @@ +log <- file(snakemake@log[[1]], open='wt') +sink(file=log, type='message') +sink(file=log, type='output') + +source("utils/plot-clustering.R") +plot.clustering(inputfile = snakemake@input[["sv_calls"]], bin.bed.filename = snakemake@input[["binbed"]], position.outputfile = snakemake@output[["position"]], chromosome.outputfile = snakemake@output[["chromosome"]])