FROM rocker/r-ver:3.4.3 MAINTAINER Sascha Meiers meiers@embl.de LABEL version="1.1" LABEL mosaicatcher_version="0.3.1-dev" # LABEL strandphaser_version="24eabf99a15c2ab959f7c5667cc22ef994cd0fc5" LABEL strandphaser_version="8011371c3a2f2d3a92116eb9df657b42d0b5e5b4" LABEL description="Required software dependencies for the MosaiCatcher pipeline (https://github.com/friendsofstrandseq/pipeline) to be used from within Snakemake." # Install basic required packages RUN apt-get update \ && apt-get install --no-install-recommends -y \ libssl-dev \ libcurl4-openssl-dev \ libboost-program-options1.62.0 \ libboost-program-options1.62-dev \ libboost-random1.62-dev \ libboost-system1.62.0 \ libboost-system1.62-dev \ libboost-filesystem1.62.0 \ libboost-filesystem1.62-dev \ libboost-iostreams1.62.0 \ libboost-iostreams1.62-dev \ libboost-date-time1.62.0 \ libboost-date-time1.62-dev \ zlib1g-dev \ libbz2-dev \ liblzma-dev \ libxml2-dev \ gawk \ bcftools=1.3.1-1+b1 \ samtools=1.3.1-3 \ && apt-get autoremove -y \ && rm -rf /var/lib/apt/lists/* \ && ln -s $(which python3) /usr/local/sbin/python # Install basic R packages from a fixed R version (MRAN) RUN Rscript -e "install.packages(c( \ 'assertthat', \ 'dplyr', \ 'data.table', \ 'stringr', \ 'ggplot2', \ 'cowplot', \ 'devtools', \ 'reshape2', \ 'doParallel', \ 'foreach'))" # Install StrandPhaseR RUN Rscript -e "source('http://bioconductor.org/biocLite.R'); \ biocLite('BSgenome', ask=F); \ biocLite('BSgenome.Hsapiens.UCSC.hg38', ask=F); \ devtools::install_github('daewoooo/StrandPhaseR@8011371c3a2f2d3a92116eb9df657b42d0b5e5b4', dependencies = NA);" \ && rm -rf /usr/local/lib/R/site-library/BSgenome.Hsapiens.UCSC.hg38/extdata/single_sequences.2bit # Install FreeBayes RUN apt-get update \ && BUILD_DEPS="git" \ && apt-get install --no-install-recommends -y $BUILD_DEPS \ && git clone --recursive git://github.com/ekg/freebayes.git \ && cd freebayes \ && make \ && cd \ && ln -s /freebayes/bin/freebayes /usr/local/sbin/freebayes \ && apt-get remove -y $BUILD_DEPS \ && apt-get autoremove -y \ && rm -rf /var/lib/apt/lists/* # Install MosaiCatcher (and clean up afterwards) RUN apt-get update \ && BUILD_DEPS="cmake \ git" \ && apt-get install --no-install-recommends -y $BUILD_DEPS \ && git clone https://github.com/friendsofstrandseq/mosaicatcher.git \ && cd mosaicatcher \ && git checkout 0.3.1-dev \ && mkdir build \ && cd build \ && cmake ../src/ \ && cmake --build . \ && cd \ && ln -s /mosaicatcher/build/mosaic /usr/local/sbin/mosaic \ && apt-get remove -y $BUILD_DEPS \ && apt-get autoremove -y \ && rm -rf /var/lib/apt/lists/* # Install dependencies for python scripts ENV PATH="${PATH}:/miniconda/bin" RUN apt-get update \ && BUILD_DEPS="wget \ bzip2" \ && apt-get install --no-install-recommends -y $BUILD_DEPS \ && wget https://repo.anaconda.com/miniconda/Miniconda3-latest-Linux-x86_64.sh \ && chmod +x Miniconda3-latest-Linux-x86_64.sh \ && ./Miniconda3-latest-Linux-x86_64.sh -b -p /miniconda \ && rm -rf Miniconda3-latest-Linux-x86_64.sh \ && apt-get remove -y $BUILD_DEPS \ && apt-get autoremove -y \ && rm -rf /var/lib/apt/lists/*