Commit 8f7a9e43 authored by Vallo Varik's avatar Vallo Varik
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Add task 5

parent f8cc12e5
......@@ -67,3 +67,12 @@ possible.
-1]`. Input [data](doc/tasks/03_dat.csv) (the same as in step 3 above).
And if you now plot everything exactly as in step 3 above, except having OD on y-axis, here's what [output](doc/tasks/04_out.pdf) should look like.
5. Constrain the OD at limit of detection. You might have noticed on the
previous plot that some of the growth curves start at very low values. In
fact, some of the ODs ended up negative. This is because the values are
actually lower bound by limit of detection (LOD). Experience tells that at
OD~595~ with 30 µL/well in LB, the limit of detection is ~0.03. So the
final step for deriving background subtracted ODs is to constrain OD at
0.03. Multiple ways are again possible, I would go for `ifelse` statement.
Here's what the resulting [output](doc/tasks/05_out.pdf) plot should look
like.
......@@ -53,3 +53,13 @@ to use the former whenever possible.
you now plot everything exactly as in step 3 above, except having OD
on y-axis, here’s what [output](doc/tasks/04_out.pdf) should look
like.
2. Constrain the OD at limit of detection. You might have noticed on
the previous plot that some of the growth curves start at very low
values. In fact, some of the ODs ended up negative. This is because
the values are actually lower bound by limit of detection (LOD).
Experience tells that at OD<sub>595</sub> with 30 µL/well in LB, the
limit of detection is ~0.03. So the final step for deriving
background subtracted ODs is to constrain OD at 0.03. Multiple ways
are again possible, I would go for `ifelse` statement. Here’s what
the resulting [output](doc/tasks/05_out.pdf) plot should look like.
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