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### Welcome to a Wiki page of FCS pipeline!
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Here we explain how to install and use Fluorescence Correlation Spectroscopy (FCS) pipeline. The FCS pipeline is a FCS-based calibrated imaging analysis software for the interactive and automated processing of FCS data.
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Here we explain how to install and use Fluorescence Correlation Spectroscopy (FCS) pipeline. The FCS pipeline is an FCS-based calibrated image analysis software for the interactive and automated processing of FCS data.
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#### 1. [Installation](#installation)
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#### 2. [Structure of workflow](#structure-of-workflow)
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| ... | ... | @@ -10,7 +10,7 @@ Here we explain how to install and use Fluorescence Correlation Spectroscopy (FC |
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1. Download the latest [KNIME Analytics Platform](https://www.knime.com/downloads). It is available for Windows, Linux, and Mac OS X.
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> Take a look on [Getting Started](https://www.knime.com/knime) for a brief introduction into KNIME Analytics Platform.
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2. Install the [KNIME Python Integration](https://docs.knime.com/2018-12/python_installation_guide/index.html) to be used with KNIME Analytics Platform.
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3. Save and unzip [fcs_pipeline.zip](https://git.embl.de/halavaty/fcs-pipelines-vitali-vistunou) in knime-workspace directory (created after KNIME installation) in your PC. fcs_pipeline directory includes all files needed for fcs pipeline to work.
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3. Save and unzip [fcs_pipeline.zip](https://git.embl.de/halavaty/fcs-pipelines-vitali-vistunou) in knime-workspace directory that was created after KNIME installation. fcs_pipeline directory includes all files needed for fcs pipeline to work.
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4. Install [Fluctuation Analyzer (FA) software](http://fluctuations.de/downloads.html) provided by Malte Wachsmuth.
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#### Structure of workflow
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- two user inputs:
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| ... | ... | @@ -57,7 +57,7 @@ info.csv is generated inside the main user input. This is the main output with a |
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info.csv includes:
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* diffusion coefficient of dye and confocal volume
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* the names of directories and files with fcs data
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* the names of directories and files with FCS data
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* path to the main directory
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* calibration plot parameters and its errors
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* k - parameter that shows the intersection rate of intensity ranges of POI and FP
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