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#### 4. [Interactive Visualisation of Calibration Plot](#interactive-visualisation-of-calibration-plot)
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#### 5. [Output files](#output-files)
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#### Installation
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1. Download the latest [KNIME Analytics Platform] It is available for Windows, Linux, and Mac OS X.(https://www.knime.com/downloads).
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1. Download the latest [KNIME Analytics Platform](https://www.knime.com/downloads). It is available for Windows, Linux, and Mac OS X.
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> Take a look on [Getting Started](https://www.knime.com/knime) for a brief introduction into KNIME Analytics Platform.
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2. Install the [KNIME Python Integration](https://docs.knime.com/2018-12/python_installation_guide/index.html) to be used with KNIME Analytics Platform.
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3. Save and unzip [fcs_pipeline.zip](https://git.embl.de/halavaty/fcs-pipelines-vitali-vistunou) in knime-workspace directory (created after KNIME installation) in your PC. fcs_pipeline directory includes all files needed for fcs pipeline to work.
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> For the execution of all nodes at one time, click on the green bottom (Execute all executable nodes) at the top of the KNIME window or just press a shortcut: Shift+F7.
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#### Interactive Visualisation of Calibration Plot
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1. Pick the point of interest in the calibration plot window to see fluctuation and correlation data from respective FCS point.
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1. Pick the point of interest in the calibration plot window to see fluctuation and correlation data from respective FCS position.
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2. Check the statistics parameters and level of bleaching at the headings of plots.
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2. The menu of the plot windows can be used to:
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* Save the image of the plot
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