| ... | @@ -56,13 +56,13 @@ The block provides visualization of FCSpipelineEMBL_KNIME outputs. |
... | @@ -56,13 +56,13 @@ The block provides visualization of FCSpipelineEMBL_KNIME outputs. |
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- Perform [correlations calculation and correction](https://git.embl.de/grp-ellenberg/FCSAnalyze/-/wikis/Fa_Load_and_Correlate)
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- Perform [correlations calculation and correction](https://git.embl.de/grp-ellenberg/FCSAnalyze/-/wikis/Fa_Load_and_Correlate)
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- Perform [fitting of correlations with ACF](https://git.embl.de/grp-ellenberg/FCSAnalyze/-/wikis/Fa_fit_fcs)
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- Perform [fitting of correlations with ACF](https://git.embl.de/grp-ellenberg/FCSAnalyze/-/wikis/Fa_fit_fcs)
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<br>
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- Go to save, export and report step, click FA format to save corresponding res files (**mFP.res** and **POI.res**) and save (Export all traces button) fluctuation traces with ACF fitting curves for POI and FP.
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- Go to save, export and report step, click FA format to save corresponding res files (**mFP.res** and **POI.res**) and save (Export all traces button) fluctuation traces with ACF fitting curves for POI and FP.
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#### 3. Calculate the effective confocal volume:
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#### 3. Calculate the effective confocal volume:
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- Follow the same steps described in bullet point 2 to obtain **dye.res**
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- Follow the same steps described in bullet point 2 to obtain **dye.res**
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<br>
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**or**<br>
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**or**
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- If the effective confocal was measured without FA*, the user can specify this volume in fl in the main user input. This volume will be then used to calculate concentrations for the calibration plot. The ways to obtain the effective confocal volume outside the FCSpipelineEMBL_KNIME can be found in [technical details](https://git.embl.de/grp-almf/FCSpipelineEMBL_KNIME/-/wikis/technical-details).
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- If the effective confocal was measured without FA*, the user can specify this volume in fl in the main user input. This volume will be then used to calculate concentrations for the calibration plot. The ways to obtain the effective confocal volume outside the FCSpipelineEMBL_KNIME can be found in [technical details](https://git.embl.de/grp-almf/FCSpipelineEMBL_KNIME/-/wikis/technical-details).
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| ... | @@ -101,7 +101,7 @@ The main output node that provides interactive visualization of the calibration |
... | @@ -101,7 +101,7 @@ The main output node that provides interactive visualization of the calibration |
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#### 9. Quality Check <br>
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#### 9. Quality Check <br>
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* In the plot parameters input, list the points that haven't passed Quality Check* <br><br>
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* In the plot parameters input, list the points that haven't passed Quality Check* <br>
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**Important note**: The Standart Quality check does **not guarantee** to remove all "bad" fluctuations. Thus, we recommend going through calibration points and remove these points manually. To delete the points in the calibration plot, specify the numbers from the annotations of corresponding points in the plot parameters input (the field: points to delete).
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**Important note**: The Standart Quality check does **not guarantee** to remove all "bad" fluctuations. Thus, we recommend going through calibration points and remove these points manually. To delete the points in the calibration plot, specify the numbers from the annotations of corresponding points in the plot parameters input (the field: points to delete).
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* Reexecute the Python View node with a calibration plot
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* Reexecute the Python View node with a calibration plot
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