... | ... | @@ -3,7 +3,7 @@ Here we explain how to install and use the FCSpipelineEMBL_KNIME. |
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FCSpipelineEMBL_KNIME is a software designed for automated processing: only user inputs and visualization of data steps (see green frames) need the user's supervision. The pipeline allows the interactive inspection of results and offers the opportunity to customize the workflow by modifying the source code of individual KNIME nodes. In addition, KNIME provides the flexibility to expand the existing workflow for specific project needs.
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Explanations of **technical details** and **calculations** in FCSpipelineEMBL_KNIME calculations can be found [here](technical details)
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Explanations of **technical details** and **calculations** in FCSpipelineEMBL_KNIME can be found [here](technical details)
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#### 1. [Installation](installation)
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#### 2. [Structure of workflow](#structure-of-workflow)
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... | ... | @@ -22,7 +22,7 @@ CPM = Counts per molecule<br> |
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FP = Fluorescent protein used for FCS calibration (such as monomeric GFP)<br>
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QC - Quality Check
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![Gitlab_WF_ar](uploads/5dc4770df3256fea88df4b1467458336/Gitlab_WF_ar.jpg)
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![Gitlab_WF_ar](uploads/1515239bf88e977a0eaf09129669faf3/Gitlab_WF_ar.jpg)
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#### I - WT processing block
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The WT processing block calculates the background offset for the correction step in the FA. See [the procedure](#procedure) below.
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