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### Welcome to a Wiki page of FCS pipeline!
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#### 1. [Installation](Installation)
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#### 2. Structure of workflow:
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#### 1. [Installation](#installation)
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#### 2. [Structure of workflow](#structure-of-workflow)
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#### 3. [Prosedure](#prosedure)
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#### Installation
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1. Download the latest [KNIME Analytics Platform](https://www.knime.com/downloads).
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2. Install the [KNIME Python Integration](https://docs.knime.com/2018-12/python_installation_guide/index.html) to be used with KNIME Analytics Platform.
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> Take a look on [Getting Started](https://www.knime.com/knime) for a brief introduction into KNIME Analytics Platform.
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3. Save the [FCS-pipeline workflow](https://git.embl.de/halavaty/fcs-pipelines-vitali-vistunou) in knime workspace directory in your PC.
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#Structure of workflow
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- two user inputs:
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- main user input
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- plot parameters input
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- data processing nodes (joiner, python scripts)
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- visualisation node (Python View)
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![fcs_pipeline workflow](uploads/2c5fb65b19810368bcee452f8d6ea874/workflow.png)
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#### 3. Prosedure:
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#### Prosedure
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1. Prepare the following [structure of files](structure of files) which contains:
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* Output data from FCS experiments: .czi, .fcs, .row files produced by [ZEN software](https://www.zeiss.com/microscopy/int/products/microscope-software/zen.html)
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* Output files from [Fluctuation Analyzer (FA) software](http://fluctuations.de/downloads.html) provided by Malte Wachsmuth
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