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Update Home authored by Vitali Vistunou's avatar Vitali Vistunou
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...@@ -25,7 +25,8 @@ Here we explain how to install and use Fluorescence Correlation Spectroscopy (FC ...@@ -25,7 +25,8 @@ Here we explain how to install and use Fluorescence Correlation Spectroscopy (FC
- Table View (concentrations of POI) - Table View (concentrations of POI)
- Two Image Viewers (loaded images with info and concentration maps) - Two Image Viewers (loaded images with info and concentration maps)
![work](uploads/aefdca282c88e5685b595f611051e01c/work.png) ![eeffe](uploads/66713426afc1b365482a12d55d6bc87e/eeffe.png)
#### Procedure #### Procedure
1. This step can be omitted. Process a WT data with FA to obtain WT.res. Only loading and saving data steps are needed. Fill a WT user input and execute Python Source node. Use returned offset value for correction steps in further FA sessions for FP and POI. 1. This step can be omitted. Process a WT data with FA to obtain WT.res. Only loading and saving data steps are needed. Fill a WT user input and execute Python Source node. Use returned offset value for correction steps in further FA sessions for FP and POI.
2. Perform three separate sessions of FA analysis: for FP, for POI, and for dye. Do all intermediate FA steps (correlations calculation, correction, fitting of correlations with ACF) to obtain mFP.res, POI.res, and dye.res. A detailed explanation of FA analysis procedure can be found [here](https://git.embl.de/grp-ellenberg/FCSAnalyze/-/wikis/home). 2. Perform three separate sessions of FA analysis: for FP, for POI, and for dye. Do all intermediate FA steps (correlations calculation, correction, fitting of correlations with ACF) to obtain mFP.res, POI.res, and dye.res. A detailed explanation of FA analysis procedure can be found [here](https://git.embl.de/grp-ellenberg/FCSAnalyze/-/wikis/home).
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