... | @@ -46,9 +46,6 @@ Here we explain how to install and use FCS-calibrated image analysis pipeline. T |
... | @@ -46,9 +46,6 @@ Here we explain how to install and use FCS-calibrated image analysis pipeline. T |
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* Using [FCSFitM](https://git.embl.de/grp-ellenberg/FCSAnalyze/-/wikis/FCSFitM) provided by Antonio. In this case, users need to specify the volume in the main user input returned by FCSFitM (you can find it in focalVolume.txt). This way **requires the installation of additional software**.
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* Using [FCSFitM](https://git.embl.de/grp-ellenberg/FCSAnalyze/-/wikis/FCSFitM) provided by Antonio. In this case, users need to specify the volume in the main user input returned by FCSFitM (you can find it in focalVolume.txt). This way **requires the installation of additional software**.
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* Other strategies as described in this [article](https://www.picoquant.com/images/uploads/page/files/7351/appnote_quantfcs.pdf). After you received the value of effective confocal volume you can add it into main user input.
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* Other strategies as described in this [article](https://www.picoquant.com/images/uploads/page/files/7351/appnote_quantfcs.pdf). After you received the value of effective confocal volume you can add it into main user input.
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The explanation of every parameter can be found in a KMIME description menu of the main user input.
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![input1](uploads/a4c9df72fe2acfc0693cc9b274e0fba6/input1.png)
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![input1](uploads/a4c9df72fe2acfc0693cc9b274e0fba6/input1.png)
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5. Fill a plot parameters input. The explanation of every parameter can be found in a KMIME description menu of the plot parameters input.
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5. Fill a plot parameters input. The explanation of every parameter can be found in a KMIME description menu of the plot parameters input.
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