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Examples of files are [here](https://git.embl.de/halavaty/fcs-pipelines-vitali-vistunou)( (py36.bat and py35.sh)
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5. Save [fcs_pipeline.zip](https://git.embl.de/halavaty/fcs-pipelines-vitali-vistunou) on your computer and unzip it in knime-workspace directory.
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> fcs_pipeline directory includes all files needed for the pipeline to work.
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6. Install [Fluctuation Analyzer (FA) software](http://fluctuations.de/downloads.html) provided by Malte Wachsmuth. In case LabVIEW error, please install [.NET Framework 3.5](https://www.microsoft.com/ru-ru/download/details.aspx?id=21)
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#### Structure of workflow
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* calibration plot parameters and its errors
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* k - parameter that shows the intersection rate of intensity ranges of POI and FP
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![wh](uploads/ebd9f7de8c9c6238e062c43d6d100114/wh.png)
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2. **calibration.csv**
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Here you can find the concentrations of POI calculated using calibration plot. Check the k-value in the info.csv after execution. k > 1 leads to less confidence in the determination of POI concentration.
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