... | @@ -19,7 +19,15 @@ CPM = Counts per molecule<br> |
... | @@ -19,7 +19,15 @@ CPM = Counts per molecule<br> |
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FP = Fluorescent protein used for FCS calibration (such as monomeric GFP)<br>
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FP = Fluorescent protein used for FCS calibration (such as monomeric GFP)<br>
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QC - Quality Check
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QC - Quality Check
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**Important note**: FCSpipelineEMBL_KNIME is an automated software. Only user inputs and visualization of data steps (see green frames) need the user's supervision.
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**Important note**: FCSpipelineEMBL_KNIME is an automated software. Only user inputs and visualization of data steps (see green frames) need the user's supervision.
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Before opening KNIME, we recommend to open Anaconda Navigator and check the environment. In the Navigator, go to environments and choose:
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The input of the individual nodes can be typed in and modified by "right click" on the node and choosing "configure". Alternatively, nodes can be opened by "double click" on the node.
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The source code in the individual nodes, also in the processing nodes, becomes visible by "double clicking" on the node. If needed, the scripts can be modified by the user in Python or Java.
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To run a node, right click on the node and choose "execute"
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![wf3](uploads/3e13219973e4aabd25390e25c2c0e788/wf3.png)
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![wf3](uploads/3e13219973e4aabd25390e25c2c0e788/wf3.png)
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... | @@ -30,15 +38,15 @@ The WT processing block calculates the background offset for the correction step |
... | @@ -30,15 +38,15 @@ The WT processing block calculates the background offset for the correction step |
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#### II - main user input
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#### II - main user input
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The main user input contains general parameters of the FCSpipelineEMBL_KNIME workflow. The explanation of every parameter can be found on the [input parameters](https://git.embl.de/grp-almf/FCSpipelineEMBL_KNIME/-/wikis/input-parameters) page or in a KMIME description menu of the main user input.
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The main user input contains the data input path and general parameters of the FCSpipelineEMBL_KNIME workflow. The explanation of every parameter can be found on the [input parameters](https://git.embl.de/grp-almf/FCSpipelineEMBL_KNIME/-/wikis/input-parameters) page or in a KMIME description menu of the main user input.
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#### III - plot parameters input
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#### III - plot parameters input
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The plot parameters input contains a calibration plot and QC parameters. For parameter description, go to the [input parameters](https://git.embl.de/grp-almf/FCSpipelineEMBL_KNIME/-/wikis/input-parameters) page.
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The plot parameters input contains a calibration plot and QC parameters. For parameter description, please go the [input parameters](https://git.embl.de/grp-almf/FCSpipelineEMBL_KNIME/-/wikis/input-parameters) page.
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#### IV - visualization block
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#### IV - visualization block
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The block provides visualization of FCSpipelineEMBL_KNIME outputs.
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The block provides visualization of FCSpipelineEMBL_KNIME outputs and saving of results in output folder.
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# Procedure
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# Procedure
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