| ... | ... | @@ -26,7 +26,7 @@ QC - Quality Check |
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#### I - WT processing block
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The WT processing block calculates the background offset for the correction step in the FA. See procedure((#procedure)) below.
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The WT processing block calculates the background offset for the correction step in the FA. See [the procedure](#procedure) below.
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#### II - main user input
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| ... | ... | @@ -56,13 +56,13 @@ The block provides visualization of FCSpipelineEMBL_KNIME outputs. |
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- Perform [correlations calculation and correction](https://git.embl.de/grp-ellenberg/FCSAnalyze/-/wikis/Fa_Load_and_Correlate)
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- Perform [fitting of correlations with ACF](https://git.embl.de/grp-ellenberg/FCSAnalyze/-/wikis/Fa_fit_fcs)
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<br><be>
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<br>
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- Go to save, export and report step, click FA format to save corresponding res files (**mFP.res** and **POI.res**) and save (Export all traces button) fluctuation traces with ACF fitting curves for POI and FP.
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#### 3. Calculate the effective confocal volume:
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- Follow the same steps described in bullet point 2 to obtain **dye.res**
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<be><br>
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**or**<be><br>
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<br>
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**or**<br>
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- If the effective confocal was measured without FA*, the user can specify this volume in fl in the main user input. This volume will be then used to calculate concentrations for the calibration plot. The ways to obtain the effective confocal volume outside the FCSpipelineEMBL_KNIME can be found in [technical details](https://git.embl.de/grp-almf/FCSpipelineEMBL_KNIME/-/wikis/technical-details).
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