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With the adaptive feedback microscopy capability of MyPiC the user can combine online image analysis and image acquisition to perform:
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With the adaptive feedback microscopy capability of MyPiC the user can combine online image analysis and image acquisition to perform:
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* Detection of rare events and start a different imaging protocol
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* Detection of rare events and start a different imaging protocol
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* Automated FCS and imaging experiments
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* Automated FCS and imaging experiments
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* Time-lapse FCS measurements
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* Time-lapse FCS measurements
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* Cell tracking
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* Cell tracking
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* Fluorescence recovery after photobleaching (FRAP) experiments
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* Fluorescence recovery after photobleaching (FRAP) experiments
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Images are acquired by the microscope and then processed by an image analysis program that is monitoring the arrival of new images to be analyzed. After the image has been analyzed commands are sent to the microscope in order to start a different pipeline or update the XYZ position for tracking an object in space and time. To start a different pipeline upon event detection the settings for the [Trigger1 and/or Trigger2](#trigger) must be defined.
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Images are acquired by the microscope and then processed by an image analysis program that is monitoring the arrival of new images to be analyzed. After the image has been analyzed commands are sent to the microscope in order to start a different pipeline or update the XYZ position for tracking an object in space and time. To start a different pipeline upon event detection the settings for [Trigger1 and/or Trigger2](#trigger) must be defined.
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The communication between the image analysis software is through the windows registry, thus any program that can read and write to the windows registry can be used. An example of an application using ImageJ for online image analysis can be found in
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The communication between the image analysis software is through the windows registry, thus any program that can read and write to the windows registry can be used. An example of an application using ImageJ for online image analysis can be found in
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