... | ... | @@ -25,7 +25,7 @@ Image-derived data are expected to be in table format with data points in rows a |
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To link data points to images, the table should include one column with the **path to image files relative to the image root directory** and each cell of this column must reference only one file. Using the example above, the image root directory is 'screen\_images' and therefore the table column for data points associated with image W001-P001-Z000-T0000-s1234-Cy3.tif should contain the relative path 'plate1_replicate1/well001/W001-P001-Z000-T0000-s1234-Cy3.tif'. There can be multiple columns with links to images but only two can be used simultaneously in the IDE.
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The IDE references ROIs by the **coordinates of an anchor point** (e.g. the ROI centre) therefore there should be a column for each of the relevant coordinates: x, y and either z or t. Coordinates (x,y) must be in pixels relative to the top left corner of the image (which is pretty much the standard for image analysis software).
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For high-throughput microscopy data, the table should have separate columns for plate and well identifiers. Wells must be identified either by indices starting from 1 in the top left corner of the plate and incremented by row from left to right or by row/column coordinates with rows identified by letters and columns by numbers with well A1 in the top left corner of the plate.
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