| ... | ... | @@ -44,7 +44,7 @@ The block provides visualization of FCSpipelineEMBL_KNIME outputs. |
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**Imporatant:** FCSpipelineEMBL_KNIME deals with the outputs of Fluctuation Analyzer. A detailed explanation of FA analysis procedure including [correlations calculation](https://git.embl.de/grp-ellenberg/FCSAnalyze/-/wikis/Fa_Load_and_Correlate) and [fitting](https://git.embl.de/grp-ellenberg/FCSAnalyze/-/wikis/Fa_fit_fcs) can be found on the Wiki page of FCS-calibrated imaging pipeline developed by Antonio Politi.
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#### 1. Process a WT data with FA:
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#### 1. Process WT FCS data with FA:
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- Perform [import, correction, and export steps in FA](https://git.embl.de/grp-ellenberg/FCSAnalyze/-/wikis/Fa_Load_and_Correlate)
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| ... | ... | @@ -52,13 +52,13 @@ The block provides visualization of FCSpipelineEMBL_KNIME outputs. |
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- Fill the WT user input and execute Python Source node. Use returned offset value (right-click -> Table) for correction steps in further FA sessions for FP and POI.
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#### 2. Perform 2 separate FA analysis sessions for POI and FP data:
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#### 2. Perform 2 separate FA analysis sessions for FCS data on POI and FP:
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- Perform [correlations calculation and correction](https://git.embl.de/grp-ellenberg/FCSAnalyze/-/wikis/Fa_Load_and_Correlate)
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- Perform [fitting of correlations with ACF](https://git.embl.de/grp-ellenberg/FCSAnalyze/-/wikis/Fa_fit_fcs)
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- Go to save, export and report step, click FA format to save corresponding res files (**mFP.res** and **POI.res**) and save (Export all traces button) fluctuation traces with ACF fitting curves for POI and FP.
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#### 3. Calculate the effective confocal volume:
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#### 3. Calculate the effective confocal volume by processing FCS data on the Dye:
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- Follow the same steps described in bullet point 2 to obtain **dye.res**
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**or**
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