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# <img src = './images/fcsrunner_icon.png'> FcsRunner
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# <img src = './images/fcsrunner_icon.png'> FcsRunner
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... | @@ -62,9 +63,9 @@ The macro allows to acquire FCS measurements at different stage positions. For e |
... | @@ -62,9 +63,9 @@ The macro allows to acquire FCS measurements at different stage positions. For e |
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FCSRunner commands | Explanations |
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FCSRunner commands | Explanations
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-----| --------
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:-----------------------------------------:|:---------
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<img src="./images/fcsRunner.PNG" width = "400px"> | 1. Specify the number and location/type of `FCS points per object` . This information will be written in the [xml file](#xmlfile). <br/> 2. In the **FCS Positions** frame the user can `Add` FCS points to the current stage position. The other buttons will `Remove` an highlighted point or `Remove All` FCS points. In order to simplify the mapping of image and FCS position the macro forces the FCS positions to be in the center of an image pixel. <br/> 3. In the **Stage Positions** frame the user can `Add` the current stage (X,Y) and focus wheel (Z) position to the experiment. The user can `Remove` an highlighted position or `Remove All` positions. The `Update` button will update the highlighted position to the current stage (X,Y) and focus wheel (Z) position. With a double click the microscope move to the highlighted position. <br/> 4. If `One image per object` is checked then the number per FCS points at the each position does not exceed the total number of points per object. <br/> 5. Specify output directory, file format, and base file name (an optional prefix to the file name). <br/> 6. The 3 top buttons start a measurement the last stops a measurement. The difference between buttons is the order in which FCS and imaging are executed <br/> 7. Total number of FCS measurements for all positions
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<img src="./images/fcsRunner.PNG" width = "2000px"> | 1. Specify the number and location/type of `FCS points per object` . This information will be written in the [xml file](#xmlfile). <br/> 2. In the **FCS Positions** frame the user can `Add` FCS points to the current stage position. The other buttons will `Remove` an highlighted point or `Remove All` FCS points. In order to simplify the mapping of image and FCS position the macro forces the FCS positions to be in the center of an image pixel. <br/> 3. In the **Stage Positions** frame the user can `Add` the current stage (X,Y) and focus wheel (Z) position to the experiment. The user can `Remove` an highlighted position or `Remove All` positions. The `Update` button will update the highlighted position to the current stage (X,Y) and focus wheel (Z) position. With a double click the microscope move to the highlighted position. <br/> 4. If `One image per object` is checked then the number per FCS points at the each position does not exceed the total number of points per object. <br/> 5. Specify output directory, file format, and base file name (an optional prefix to the file name). <br/> 6. The 3 top buttons start a measurement the last stops a measurement. The difference between buttons is the order in which FCS and imaging are executed <br/> 7. Total number of FCS measurements for all positions
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