... | ... | @@ -5,10 +5,9 @@ LocScale uses reference amplitudes from a refined atomic model to locally scale |
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For sharpening LocScale requires the unfiltered, unsharpened EM reconstruction (or half maps) along with a reference map computed from the atomic model. Optionally, a mask may be applied to speed up the computation.
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##### __Please follow these [instructions](model_map_generation) to generate your model-based reference map with B-factor weighted structure factors using electron form factors.__
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<br>
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<br>
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Requires `sparx` , `EMAN2` , (`mpi4py`)
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```
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*** Computes contrast-enhanced cryo-EM potential maps by local amplitude scaling ***
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... | ... | @@ -34,4 +33,6 @@ optional arguments: |
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```
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__Window size__: We find that a window size of approximately (7 * average_map_resolution_in_Å)/pixel_size typically works well. |
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__Window size__: We find that a window size of approximately (7 * average_map_resolution_in_Å)/pixel_size typically works well.
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__Computing time__: We strongly recommend to run the parallelized version of LocScale using MPI. Computing LocScale maps on a a single node can be (very) slow. In comparison, computation of a LocScale map for EMD-5778 takes about 4 min on 50 CPUs using MPI. |