LocScale is a reference-based local amplitude scaling tool using prior model information to improve contrast of cryo-EM density maps. It can be used as an alternative to other commonly used map sharpening methods.
To reliably reflect the required level of local sharpening and local resolution variation in the target map, atomic B-factors of the reference model should have been refined using appropriate protocols prior to scaling. It is also possible to use LocScale for local sharpening without a model in cases where an atomic model is not available. The method is described in more detail here .
To run on multiple CPUs in parallel, LocScale uses MPI for distributed-memory parallelisation. Execution of the MPI-parallelized version requires installation of an MPI implementation (e.g. openMPI) on your system.
https://git.embl.de/jakobi/LocScale/tree/master/download) for download.The program and associated utils are available [here] (
Please consult the Wiki pages for instructions on:
 Jakobi et al. eLife 6:e27131 (2017)