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LocScale is a reference-based local amplitude scaling tool using prior model information to improve contrast of cryo-EM density maps. It can be used as an alternative to other commonly used map sharpening methods.
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To reliably reflect the required level of local sharpening and local resolution variation in the target map, atomic B-factors of the reference model should have been refined using appropriate protocols prior to scaling. The method is described in more detail in a [bioRxiv](https://doi.org/10.1101/121913) preprint [1].
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To reliably reflect the required level of local sharpening and local resolution variation in the target map, atomic B-factors of the reference model should have been refined using appropriate protocols prior to scaling. The method is described in more detail here [1].
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## References
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[[1]](https://doi.org/10.1101/121913) Jakobi et al. bioRxiv 121913 (2017) |
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[[1]](https://doi.org/10.7554/eLife.27131) Jakobi et al. eLife 6:e27131 (2017) |
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