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Bernd Klaus
htm_course_2016
Commits
0b9346b3
Commit
0b9346b3
authored
8 years ago
by
Andrzej Oles
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Final fixes to the vignette
parent
c0013eff
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Tutorial_HTM_2016.Rmd
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Tutorial_HTM_2016.Rmd
Tutorial_HTM_2016.html
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Tutorial_HTM_2016.Rmd
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---
---
title: "Visual Exploration of High-Throughput-Microscopy Data"
title: "Visual Exploration of High-Throughput-Microscopy Data"
author: "Bernd Klaus, Andrzej Ole
s
, Mike Smith"
author: "Bernd Klaus, Andrzej Ole
ś
, Mike Smith"
date: "`r doc_date()`"
date: "`r doc_date()`"
bibliography: HTM_2016.bib
bibliography: HTM_2016.bib
output:
output:
...
@@ -59,7 +59,6 @@ each single cell. These classification results have been obtained using a machin
...
@@ -59,7 +59,6 @@ each single cell. These classification results have been obtained using a machin
learning algorithm based on the original image features. The data produced is similar
learning algorithm based on the original image features. The data produced is similar
to the one in @Neumann_2010: Each cell is classified into a mitotic phenotype class.
to the one in @Neumann_2010: Each cell is classified into a mitotic phenotype class.
<!--  -->
# Annotation import
# Annotation import
...
@@ -77,7 +76,7 @@ head(plate_map)
...
@@ -77,7 +76,7 @@ head(plate_map)
# Importing the raw data
# Importing the raw data
We will now import the raw data. This data is stored in a variant of the [HDF5 format](https://en.wikipedia.org/wiki/Hierarchical_Data_Format) called
We will now import the raw data. This data is stored in a variant of the [HDF5 format](https://en.wikipedia.org/wiki/Hierarchical_Data_Format) called
[
"CellH5
"
](http://www.cellh5.org/),
"
[
CellH5](http://www.cellh5.org/)
"
,
which defines a more restricted sub-format designed specifically to store data
which defines a more restricted sub-format designed specifically to store data
from high content screens. More information can be found in the paper by
from high content screens. More information can be found in the paper by
@Sommer_2013.
@Sommer_2013.
...
@@ -106,7 +105,7 @@ the screen plate in the columns and the counts for the respective classes in the
...
@@ -106,7 +105,7 @@ the screen plate in the columns and the counts for the respective classes in the
rows.
rows.
This is a typical example of a "wide" data table, where the variables
This is a typical example of a "wide" data table, where the variables
contained in the data set spread across multiple columns.
contained in the data set spread across multiple columns
(here we only show the first six ones)
.
```{r import_data_table, dependson="readCellH5"}
```{r import_data_table, dependson="readCellH5"}
raw_data <- sapply(c5_pos,
raw_data <- sapply(c5_pos,
...
@@ -115,7 +114,7 @@ raw_data <- sapply(c5_pos,
...
@@ -115,7 +114,7 @@ raw_data <- sapply(c5_pos,
table(predictions)
table(predictions)
})
})
head(
raw_data
)
raw_data
[, 1:6]
```
```
...
@@ -496,7 +495,7 @@ assembled in the R object `DNase`, which conveniently comes with base R.
...
@@ -496,7 +495,7 @@ assembled in the R object `DNase`, which conveniently comes with base R.
`conc`, the protein concentration that was used; and `density`, the
`conc`, the protein concentration that was used; and `density`, the
measured optical density.
measured optical density.
```{r figredobasicplottingwithggplot, fig.width =
3.5
, fig.height =
5
}
```{r figredobasicplottingwithggplot, fig.width =
6
, fig.height =
9
}
ggplot(DNase, aes(x = conc, y = density, color = Run)) + geom_point()
ggplot(DNase, aes(x = conc, y = density, color = Run)) + geom_point()
```
```
...
@@ -507,7 +506,7 @@ Then we told `ggplot` via the aesthetics `aes` argument which variables
...
@@ -507,7 +506,7 @@ Then we told `ggplot` via the aesthetics `aes` argument which variables
we want on the $x$- and $y$-axes, respectively and mapped the run number
we want on the $x$- and $y$-axes, respectively and mapped the run number
to the color aesthetic.
to the color aesthetic.
Finally, we stated that we want the plot to use points, by adding the result
Finally, we stated that we want the plot to use points, by adding the result
of calling the function `geom
\
_point`.
of calling the function `geom_point`.
## Principal component analysis (PCA) to for data visualization
## Principal component analysis (PCA) to for data visualization
...
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