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Christian Tischer
platy-browser-data
Commits
07e9d627
Commit
07e9d627
authored
5 years ago
by
Constantin Pape
Browse files
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Update registration mechanism and fix edu names
parent
a92a627c
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2 changed files
scripts/attributes/genes.py
+3
-10
3 additions, 10 deletions
scripts/attributes/genes.py
update_registration.py
+25
-15
25 additions, 15 deletions
update_registration.py
with
28 additions
and
25 deletions
scripts/attributes/genes.py
+
3
−
10
View file @
07e9d627
...
...
@@ -47,26 +47,19 @@ def create_auxiliary_gene_file(meds_path, out_file, return_result=False):
names_dset
=
'
gene_names
'
dset
=
'
t00000/s00/0/cells
'
# NOTE special treatment of edu will not be necessary if we add MED postfix to them again
# get all the meds in the folder
# 1.) meds
# get all the med files in the image folder
med_files
=
glob
(
os
.
path
.
join
(
meds_path
,
"
*MED.h5
"
))
gene_names
=
[
os
.
path
.
splitext
(
os
.
path
.
basename
(
f
))[
0
][:
-
4
]
for
f
in
med_files
]
# 2.) edu
edu_files
=
glob
(
os
.
path
.
join
(
meds_path
,
"
*edu*
"
))
gene_names
+=
[
os
.
path
.
splitext
(
os
.
path
.
basename
(
f
))[
0
]
for
f
in
edu_files
]
all_files
=
med_files
+
edu_files
# find out where the gene name actually starts (the 5th dash-separated word)
name_start
=
find_nth
(
gene_names
[
0
],
'
-
'
,
4
)
+
1
# cut all the preceeding prospr-... part
gene_names
=
[
name
[
name_start
:]
for
name
in
gene_names
]
num_genes
=
len
(
all
_files
)
num_genes
=
len
(
med
_files
)
assert
len
(
gene_names
)
==
num_genes
,
"
%i, %i
"
%
(
len
(
gene_names
),
len
(
num_genes
))
with
h5py
.
File
(
all
_files
[
0
],
'
r
'
)
as
f
:
with
h5py
.
File
(
med
_files
[
0
],
'
r
'
)
as
f
:
spatial_shape
=
f
[
dset
].
shape
shape
=
(
num_genes
,)
+
spatial_shape
...
...
This diff is collapsed.
Click to expand it.
update_registration.py
+
25
−
15
View file @
07e9d627
...
...
@@ -12,7 +12,7 @@ import numpy as np
import
pandas
as
pd
from
pybdv
import
make_bdv
from
scripts.files
import
copy_release_folder
,
make_folder_structure
,
make_bdv_server_file
from
scripts.files
import
copy_release_folder
,
make_folder_structure
,
make_bdv_server_file
,
get_source_names
from
scripts.files.xml_utils
import
get_h5_path_from_xml
,
write_simple_xml
from
scripts.release_helper
import
add_version
from
scripts.extension.registration
import
ApplyRegistrationLocal
,
ApplyRegistrationSlurm
...
...
@@ -41,7 +41,7 @@ def get_tags(new_tag):
return
tag
,
new_tag
def
get_out_name
(
prefix
,
name
):
def
parse_prospr
(
prefix
,
name
):
name
=
os
.
path
.
split
(
name
)[
1
]
name
=
os
.
path
.
splitext
(
name
)[
0
]
name
=
name
.
split
(
'
--
'
)[
0
]
...
...
@@ -50,9 +50,6 @@ def get_out_name(prefix, name):
# name = name.split('-')[0]
if
name
in
REGION_NAMES
:
name
=
'
-
'
.
join
([
prefix
,
'
segmented
'
,
name
])
# TODO edus shouls still have MED postfix !
elif
name
.
startswith
(
'
edu
'
):
# edus are no meds
name
=
'
-
'
.
join
([
prefix
,
name
])
else
:
name
=
'
-
'
.
join
([
prefix
,
name
,
'
MED
'
])
return
name
...
...
@@ -79,7 +76,7 @@ def copy_to_h5(inputs, output_folder):
def
apply_registration
(
input_folder
,
new_folder
,
transformation_file
,
source_prefix
,
target
,
max_jobs
):
target
,
max_jobs
,
name_parser
):
task
=
ApplyRegistrationSlurm
if
target
==
'
slurm
'
else
ApplyRegistrationLocal
tmp_folder
=
'
./tmp_registration
'
os
.
makedirs
(
tmp_folder
,
exist_ok
=
True
)
...
...
@@ -98,7 +95,7 @@ def apply_registration(input_folder, new_folder,
# output_folder = os.path.join(new_folder, 'images')
output_folder
=
os
.
path
.
join
(
tmp_folder
,
'
outputs
'
)
os
.
makedirs
(
output_folder
,
exist_ok
=
True
)
output_names
=
[
get_out_name
(
source_prefix
,
name
)
for
name
in
inputs
]
output_names
=
[
name_parser
(
source_prefix
,
name
)
for
name
in
inputs
]
outputs
=
[
os
.
path
.
join
(
output_folder
,
name
)
for
name
in
output_names
]
# update the task config
...
...
@@ -142,7 +139,7 @@ def apply_registration(input_folder, new_folder,
copy_to_h5
(
outputs
,
output_folder
)
def
update_
gene_attributes
(
new_folder
,
target
):
def
update_
prospr
(
new_folder
,
target
):
# update the auxiliaty gene volume
image_folder
=
os
.
path
.
join
(
new_folder
,
'
images
'
)
...
...
@@ -173,8 +170,12 @@ def update_gene_attributes(new_folder, target):
write_genes_table
(
seg_path
,
aux_out_path
,
out_path
,
labels
,
tmp_folder
,
target
)
# TODO we should register the virtual cells here as well
def
update_regestration
(
transformation_file
,
input_folder
,
source_prefix
,
target
,
max_jobs
,
# we should encode the source prefix and the transformation file to be used
# in a config file in the transformation folder
def
update_registration
(
transformation_file
,
input_folder
,
source_prefix
,
target
,
max_jobs
,
new_tag
=
None
):
"""
Update the prospr segmentation.
This is a special case of
'
update_patch
'
, that applies a new prospr registration.
...
...
@@ -187,6 +188,10 @@ def update_regestration(transformation_file, input_folder, source_prefix, target
max_jobs [int] - max number of jobs for computation
new_tag [str] - new version tag (default: None)
"""
prefixes
=
get_source_names
()
if
source_prefix
not
in
prefixes
:
raise
ValueError
(
"
Invalid source name %s
"
%
source_prefix
)
tag
,
new_tag
=
get_tags
(
new_tag
)
print
(
"
Updating platy browser from
"
,
tag
,
"
to
"
,
new_tag
)
...
...
@@ -198,16 +203,21 @@ def update_regestration(transformation_file, input_folder, source_prefix, target
# copy the release folder
copy_release_folder
(
folder
,
new_folder
,
exclude_prefixes
=
[
source_prefix
])
if
source_prefix
==
"
prospr-6dpf-1-whole
"
:
name_parser
=
parse_prospr
else
:
raise
NotImplementedError
# apply new registration to all files of the source prefix
transformation_file
=
os
.
path
.
abspath
(
transformation_file
)
apply_registration
(
input_folder
,
new_folder
,
transformation_file
,
source_prefix
,
target
,
max_jobs
)
# TODO we should register virtual cells here too
target
,
max_jobs
,
name_parser
)
# update gene table
update_gene_attributes
(
new_folder
,
target
)
if
source_prefix
==
"
prospr-6dpf-1-whole
"
:
update_prospr
(
new_folder
,
target
)
else
:
raise
NotImplementedError
add_version
(
new_tag
)
make_bdv_server_file
(
new_folder
,
os
.
path
.
join
(
new_folder
,
'
misc
'
,
'
bdv_server.txt
'
),
...
...
@@ -236,5 +246,5 @@ if __name__ == '__main__':
new_tag
=
args
.
new_tag
new_tag
=
None
if
new_tag
==
''
else
new_tag
update_reg
e
stration
(
args
.
transformation_file
,
args
.
input_folder
,
args
.
source_prefix
,
update_reg
i
stration
(
args
.
transformation_file
,
args
.
input_folder
,
args
.
source_prefix
,
args
.
target
,
args
.
max_jobs
,
new_tag
)
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