Further, it is often advisable to increase the time Snakemake waits for the
file system via this flag:
```
--latency-wait 60
```
Finally, of course the cluster mode can be combined with `--use-singularity`.
## Provide SNV calls
## SNP calls
The pipeline will run simple SNV calling using [samtools](https://github.com/samtools/samtools) and [bcftools](https://github.com/samtools/bcftools) on Strand-seq. If you **already have
SNV calls**, you can avoid that by entering your VCF files into the pipeline.