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Commit 0e5045c0 authored by Sascha Meiers's avatar Sascha Meiers
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Further README updates

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......@@ -158,23 +158,29 @@ In case you do not have Conda yet, it is easiest to just install
3. Run `snakemake`
## Cluster support
## Snakemake recommendations
You can ask Snakemake to submit your jobs to a HPC cluster. We provided a config
file (`cluster.json`) for this purpose, yet it might need to be adapted to your
infrastructure.
infrastructure. Here is an example command:
```
snakemake -j 100 \
--cluster-config Snake.cluster.json \
--cluster "sbatch --cpus-per-task {cluster.n} --time {cluster.time} --mem {cluster.mem}"
```
Further, it is often advisable to increase the time Snakemake waits for the
file system via this flag:
```
--latency-wait 60
```
Finally, of course the cluster mode can be combined with `--use-singularity`.
## Provide SNV calls
## SNP calls
The pipeline will run simple SNV calling using [samtools](https://github.com/samtools/samtools) and [bcftools](https://github.com/samtools/bcftools) on Strand-seq. If you **already have
SNV calls**, you can avoid that by entering your VCF files into the pipeline.
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