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Commit c56a370c authored by Tobias Marschall's avatar Tobias Marschall
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Explain Bioconda in README

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Preliminary SV calling using Strand-seq data - summarized in a [Snakemake](https://bitbucket.org/snakemake/snakemake) pipeline.
### Bioconda environment
To install the correct environment, you can use Bioconda.
1. **Install MiniConda:**
In case you do not have Conda yet, it is easiest to just install [MiniConda](https://conda.io/miniconda.html).
2. **Create environment:**
```
conda env create -n strandseqnation -f conda-environment.yml
source activate strandseqnation
```
That's it, you are ready to go.
### How to use it
1. **Install required software:**
* Install [mosaicatcher](https://github.com/friendsofstrandseq/mosaicatcher) (*currently you will need the `develop` branch*)
* Get the R-scripts from [strandsequtils](https://github.com/friendsofstrandseq/strandsequtils)
* Install BSgenome.Hsapiens.UCSC.hg38:
* Install BSgenome.Hsapiens.UCSC.hg38 (can be skipped of you use the Bioconda environment, see above):
```
source("https://bioconductor.org/biocLite.R")
biocLite('BSgenome.Hsapiens.UCSC.hg38')
```
* [Strand-Phaser](https://github.com/daewoooo/StrandPhaseR) is installed automatically
2. **Set up the configuration of the smakemake pipeline**
2. **Set up the configuration of the snakemake pipeline**
* Open `Snake.config.json` and specify the path to the executatables
(such as Mosaicatcher) and to the R scripts.
......@@ -37,7 +51,7 @@ Preliminary SV calling using Strand-seq data - summarized in a [Snakemake](https
--cluster-config Snake.cluster.json \
--cluster "???"
```
### SNV calls
The pipeline will run simple SNV calling using [samtools](https://github.com/samtools/samtools)
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