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Thomas Weber
Mosaicatcher Update
Commits
c56a370c
Commit
c56a370c
authored
7 years ago
by
Tobias Marschall
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Explain Bioconda in README
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@@ -4,20 +4,34 @@
Preliminary SV calling using Strand-seq data - summarized in a
[
Snakemake
](
https://bitbucket.org/snakemake/snakemake
)
pipeline.
### Bioconda environment
To install the correct environment, you can use Bioconda.
1.
**Install MiniConda:**
In case you do not have Conda yet, it is easiest to just install
[
MiniConda
](
https://conda.io/miniconda.html
)
.
2.
**Create environment:**
```
conda env create -n strandseqnation -f conda-environment.yml
source activate strandseqnation
```
That's it, you are ready to go.
### How to use it
1.
**Install required software:**
* Install [mosaicatcher](https://github.com/friendsofstrandseq/mosaicatcher) (*currently you will need the `develop` branch*)
* Get the R-scripts from [strandsequtils](https://github.com/friendsofstrandseq/strandsequtils)
* Install BSgenome.Hsapiens.UCSC.hg38:
* Install BSgenome.Hsapiens.UCSC.hg38
(can be skipped of you use the Bioconda environment, see above)
:
```
source("https://bioconductor.org/biocLite.R")
biocLite('BSgenome.Hsapiens.UCSC.hg38')
```
* [Strand-Phaser](https://github.com/daewoooo/StrandPhaseR) is installed automatically
2.
**Set up the configuration of the s
m
akemake pipeline**
2.
**Set up the configuration of the s
n
akemake pipeline**
* Open `Snake.config.json` and specify the path to the executatables
(such as Mosaicatcher) and to the R scripts.
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@@ -37,7 +51,7 @@ Preliminary SV calling using Strand-seq data - summarized in a [Snakemake](https
--cluster-config Snake.cluster.json \
--cluster "???"
```
### SNV calls
The pipeline will run simple SNV calling using
[
samtools
](
https://github.com/samtools/samtools
)
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