|
|
# FCSAnalyze tools
|
|
|
In this WiKi we provide examples on how to analyze and process FCS and imaging data generated on Zeiss LSM microscopes using [FCSRunner](https://git.embl.de/politi/fcsrunner) or [microscopy pipeline constructor](https://git.embl.de/politi/mypic) and [Automated FCS](https://git.embl.de/politi/adaptive_feedback_mic_fiji). The processed data can be used to generate a FCS calibration curve for converting pixel fluorescence intensities to concentrations and protein numbers.
|
|
|
In this WiKi we provide examples on how to analyze and process FCS and imaging data generated on Zeiss LSM microscopes using [FCSRunner](https://git.embl.de/politi/fcsrunner) or [MyPiC](https://git.embl.de/politi/mypic) and [Automated FCS](https://git.embl.de/politi/adaptive_feedback_mic_fiji). The processed data can be used to generate a FCS calibration curve for converting pixel fluorescence intensities in to concentrations and protein numbers.
|
|
|
|
|
|
The processing steps are
|
|
|
|
|
|
|
|
|
[**I. Generate bleach corrected correlation functions using Fluctuation Analyzer 4G**](./Fa_Load_and_Correlate)
|
|
|
|
|
|
[**II. FCSImageBroswer browses through images and FCS positions**](./Fcsimagebrowser)
|
|
|
[**II. FCSImageBrowser browses through images and FCS positions**](./Fcsimagebrowser)
|
|
|
|
|
|
[**IIIa. Fit F(C)CS data with FitFCSM**](./Fcsfitm)
|
|
|
|
... | ... | @@ -15,7 +15,7 @@ The processing steps are |
|
|
|
|
|
[**IV. FCSCalibration computes the parameters of the calibration curve**](./Fcscalibration)
|
|
|
|
|
|
[**V. FCSCalibrateImage converts fluorescence intensities to concentrations and number of proteins**](./Fcscalibrateimage)
|
|
|
[**V. FCSCalibrateImage converts fluorescence intensities in to concentrations and number of proteins**](./Fcscalibrateimage)
|
|
|
|
|
|
|
|
|
## Author
|
... | ... | |