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vertical-align : top
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vertical-align : top
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}
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}
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</style>
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</style>
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# Wiki FCSAnalyze tools
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# Wiki FCSAnalyze tools
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In this WiKi we provide examples on how to analyze and process FCS and imaging data generated on Zeiss LSM microscopes using [microscopy pipeline constructor](#https://git.embl.de/politi/mypic) or
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In this WiKi we provide examples on how to analyze and process FCS and imaging data generated on Zeiss LSM microscopes using [microscopy pipeline constructor](#https://git.embl.de/politi/mypic) or
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[FCSRunner](#https://git.embl.de/politi/fcsrunner). The processed data can be used to generate a calibration curve for converting pixel fluorescence intensities to concentrations.
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[FCSRunner](#https://git.embl.de/politi/fcsrunner). The processed data can be used to generate a calibration curve for converting pixel fluorescence intensities to concentrations.
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... | @@ -100,7 +102,10 @@ The [**Offset**](#offset) and [**Crosstalk**](#xtalk) parameters are used for co |
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* Change to `Intensity corrections` and enter the offset values for Ch1 and Ch2
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* Change to `Intensity corrections` and enter the offset values for Ch1 and Ch2
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* Calculate all corrections and save *res* table
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* Calculate all corrections and save *res* table
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* Compute crosstalk from Ch1 (to Ch2) from
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* Compute crosstalk from Ch1 (to Ch2) from
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$\frac{Interval Ch2 - Offset Ch2}{Interval Ch1 - OffsetCh1}$
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```math
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\frac{\text{IntervalCh2} - \text{OffsetCh2}}{\text{IntervalCh1} - \text{OffsetCh1}}
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```
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