... | ... | @@ -2,23 +2,25 @@ |
|
|
In this WiKi we provide examples on how to analyze and process FCS and imaging data generated on Zeiss LSM microscopes using [microscopy pipeline constructor](https://git.embl.de/politi/mypic) or
|
|
|
[FCSRunner](https://git.embl.de/politi/fcsrunner). The processed data can be used to generate a calibration curve for converting pixel fluorescence intensities to concentrations.
|
|
|
|
|
|
The order
|
|
|
|
|
|
[**Generate bleach corrected correlation functions using Fluctuation Analyzer 4G**](./FA_Load_and_Correlate)
|
|
|
|
|
|
[**FCSImageBroswer browse through image and FCS positions**](./Fcsimagebrowser)
|
|
|
[**1. Generate bleach corrected correlation functions using Fluctuation Analyzer 4G**](./FA_Load_and_Correlate)
|
|
|
|
|
|
[**Fit F(C)CS data with FitFCSM**](./Fcsfitm)
|
|
|
[**2. FCSImageBroswer browse through image and FCS positions**](./Fcsimagebrowser)
|
|
|
|
|
|
[**3a. Fit F(C)CS data with FitFCSM**](./Fcsfitm)
|
|
|
|
|
|
|
|
|
[**FCSCalibration computes the parameters of the calibration curve**](./Fcscalibration)
|
|
|
|
|
|
[**FCSCalibrateImage converts fluorescence intensities to concentrations and number of proteins**](./Fcscalibrateimage)
|
|
|
[**4. FCSCalibration computes the parameters of the calibration curve**](./Fcscalibration)
|
|
|
|
|
|
[**5. FCSCalibrateImage converts fluorescence intensities to concentrations and number of proteins**](./Fcscalibrateimage)
|
|
|
|
|
|
|
|
|
Alternatively the data fit can be performed with Fluctuation Analyzer
|
|
|
|
|
|
[**Fit F(C)CS data with Fluctuation Analyzer 4G (FA)**](./FA_Fit_FCS)
|
|
|
[**3b. Fit F(C)CS data with Fluctuation Analyzer 4G (FA)**](./FA_Fit_FCS)
|
|
|
|
|
|
|
|
|
|
... | ... | |