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# Wiki FCSAnalyze tools
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## Fluctuation Analyzer
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The Fluctuation Analyzer is a software tool for the interactive as well as automated processing of fluorescence auto- and cross-correlation spectroscopy (FCS/FCCS) data. It can read raw data, i.e., one-or two-channel photon streams, from various commercial suppliers of FCS/FCCS data acquisition equipment, organize such data in processing sessions by file-based management, calculate temporal auto- and cross-correlation functions, correct for photobleaching, cross-talk, and background signal and fit the data with appropriate model functions before saving the results. Please download Fluctuation Analyzer 4G and the user guide from https://www.embl.de/~wachsmut/downloads.html. A datailed explanation on which settings and parameters to use for the FCS calibration protocol is given [here](./fluctuationanalyzer.md). |