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In this WiKi we provide examples on how to analyze and process FCS and imaging data generated on Zeiss LSM microscopes using [microscopy pipeline constructor](https://git.embl.de/politi/mypic) or
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[FCSRunner](https://git.embl.de/politi/fcsrunner). The processed data can be used to generate a calibration curve for converting pixel fluorescence intensities to concentrations.
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The order
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The order of the processing step is
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[**1. Generate bleach corrected correlation functions using Fluctuation Analyzer 4G**](./FA_Load_and_Correlate)
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