... | ... | @@ -71,7 +71,10 @@ The macro allows to acquire FCS measurements at different stage positions. For e |
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* **FCS positions**: These are the scanner positions where the FCS measurements are acquired. They are relative to the current stage position.
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* **Stage positions**: The XY stage and Z focus position.
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> Do not move the stage or focus position when placing FCS points for a cell. If you need to correct an imaging position, remove all FCS positions, change stage and focus positions and press `Update`. Then add new FCS positions.
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> Stage Z position and FCS Z position are linked. The FCS measurement will always be with respect to the focus and stage position.
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> **BUG** When several stage positions are added some positions may end up shifted with respect to the expected location. Therefore, before launching the measurements do visit all stage positions (double click) and verify the correct positioning. Eventually update the position of a wrongly assigned stage position.
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<p align = 'center'><img src="./images/fcsRunner.PNG" width = "400px"></p>
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... | ... | @@ -82,7 +85,8 @@ The macro allows to acquire FCS measurements at different stage positions. For e |
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5. Specify output directory, file format, and base file name (an optional prefix to the file name)
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6. The 3 top buttons start a measurement, the last button stops a measurement. The difference between buttons is the order in which FCS and imaging are executed
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7. Total number of FCS measurements for all positions
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8. If available the user can use DefiniteFocus (hardware autofocus). If the box is checked, then the first Z-stage position will be used as reference for the DefiniteFocus. At every new stage position a focus stabilization step is performed.
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9.
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