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# Protein bioinformatics for beginners
## Schedule
7 - 9 November, ATC Computer lab
## Dates: 8 - 9 November
## Time: TBA
## Venue: ATC Computer lab, EMBL Heidelberg
## Trainers: Toby Gibson, Marc Gouw, Manjeet Kumar, Benjamin Lang, Malvika Sharan
## Program
**Part-1 Protein databases and sequence analysis**
1. Protein databases: Malvika
- NCBI (very quick overview)
- NCBI (quick overview)
- UniProt
- Swissprot
- Cross-refrences and link-outs
......@@ -20,46 +21,53 @@
- BLASTp, BLASTn, PSI-BLAST,
- HHPred
- HMMER
3. Multiple sequence alignments: Manjeet
- UniProt aligner
3. Multiple sequence alignments: Ben
- Muscle, Clustal omega, etc.
- EMBOSS dot-plot
- Muscle, Clustal omega
4. Other resources: Toby & Malvika
- Human portein atlas
- Antibodypedia
- EMBOSS toolkits
- Pepinfo
- Sixpack
- dotmatcher: http://emboss.bioinformatics.nl/cgi-bin/emboss/dotmatcher
- Prediction of transmembrane helices in proteins: (This is a bit misplaced)
TMHMM (http://www.cbs.dtu.dk/services/TMHMM/)
- Pepinfo
- Sixpack
- dotmatcher
**Part-2 Protein structure analysis**
* Toby's keynote: * Secondary vs tertiary structure vs protein complexes (Theory)
1. Structure database: Toby
- PDB (http://www.rcsb.org/pdb/home/home.do)
2. Structure visualization: Toby
- Motif visualization:
- Jalview, MEME, Weblogo, Chimera (https://screenshots.debian.net/package/chimera2)
3. Structure prediction: ???
- jpred (http://www.compbio.dundee.ac.uk/jpred/)
- Jalview linked tool Janet (?)
- PSIpred (?)
- Tertiary structure prediction
- I-TASSER
4. Domain databases: Ben
- InterPro,
1. Protein Structures: Toby
- Structure dtabase: PDB
- Structure visualization
- Chimera V2
- Motif visualization
- Jalview
- MEME
- Weblogo
2. Structure prediction: Malvika
- jpred
- Jalview linked tool Janet
- PSIpred
- Tertiary structure prediction: Phyre2, I-TASSER
3. Domain databases: Manjeet
- InterPro & InterProScan
- Pfam
- SCOP
- SMART?
- SUPERFAMILY?
- SMART, SUPERFAMILY
4. Prediction of transmembrane helices in proteins: Manjeet
- TMHMM
5. Intrinsically disordered region : Marc
- ELM ( http://elm.eu.org)
- IUPRED (http://iupred.enzim.hu)
- DISPROT?
- ELM
- IUPRED
- DISPROT
6. Protein-protein interaction: Malvika
- STRING (http://string-db.org)
- BioGrid?
- MINT?
- STRING
- BioGrid
- MINT
### References:
- http://www.sciencedirect.com/science/book/9788131222973
- http://molbiol-tools.ca/Protein_Chemistry.htm
- http://www.ebi.ac.uk/Tools/pfa/
### Piratenpad link: https://piratenpad.de/p/gibson_workshops
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