test_roiset.py

import unittest

import numpy as np
from pathlib import Path

from model_server.conf.testing import output_path, roiset_test_data

from model_server.base.roiset import RoiSetExportParams, RoiSetMetaParams
from model_server.base.roiset import _get_label_ids, RoiSet
from model_server.base.accessors import generate_file_accessor, InMemoryDataAccessor, write_accessor_data_to_file
from model_server.base.models import DummyInstanceSegmentationModel

class BaseTestRoiSetMonoProducts(object):

    def setUp(self) -> None:
        # set up test raw data and segmentation from file
        self.stack = generate_file_accessor(roiset_test_data['multichannel_zstack']['path'])
        self.stack_ch_pa = self.stack.get_one_channel_data(roiset_test_data['pipeline_params']['patches_channel'])
        self.seg_mask = generate_file_accessor(roiset_test_data['multichannel_zstack']['mask_path'])


class TestRoiSetMonoProducts(BaseTestRoiSetMonoProducts, unittest.TestCase):

    def _make_roi_set(self, mask_type='boxes', **kwargs):
        id_map = _get_label_ids(self.seg_mask)
        roiset = RoiSet.from_2d_obj_ids(
            self.stack_ch_pa,
            id_map,
            params=RoiSetMetaParams(
                mask_type=mask_type,
                filters=kwargs.get('filters', {'area': {'min': 1e3, 'max': 1e4}}),
                expand_box_by=(64, 2)
            )
        )
        return roiset

    def test_roi_mask_shape(self, **kwargs):
        roiset = self._make_roi_set(**kwargs)
        zmask = roiset.get_zmask()
        zmask_acc = InMemoryDataAccessor(zmask)
        self.assertTrue(zmask_acc.is_mask())

        # assert dimensionality of zmask
        self.assertEqual(zmask_acc.nz, roiset.acc_raw.nz)
        self.assertEqual(zmask_acc.chroma, 1)
        write_accessor_data_to_file(output_path / 'zmask.tif', zmask_acc)

        # mask values are not just all True or all False
        self.assertTrue(np.any(zmask))
        self.assertFalse(np.all(zmask))

        # assert non-trivial meta info in boxes
        self.assertGreater(roiset.count, 1)
        sh = roiset.get_df().iloc[1]['mask'].shape
        ar = roiset.get_df().iloc[1]['area']
        self.assertGreaterEqual(sh[0] * sh[1], ar)

    def test_roiset_from_non_zstacks(self, **kwargs):
        acc_zstack_slice = InMemoryDataAccessor(self.stack_ch_pa.data[:, :, :, 0])
        self.assertEqual(acc_zstack_slice.nz, 1)
        id_map = _get_label_ids(self.seg_mask)

        roiset = RoiSet.from_2d_obj_ids(acc_zstack_slice, id_map, params=RoiSetMetaParams(mask_type='boxes'))
        zmask = roiset.get_zmask()

        zmask_acc = InMemoryDataAccessor(zmask)
        self.assertTrue(zmask_acc.is_mask())

    def test_slices_are_valid(self):
        roiset = self._make_roi_set()
        for s in roiset.get_slices():
            ebb = roiset.acc_raw.data[s]
            self.assertEqual(len(ebb.shape), 4)
            self.assertTrue(np.all([si >= 1 for si in ebb.shape]))

    def test_rel_slices_are_valid(self):
        roiset = self._make_roi_set()
        for roi in roiset:
            ebb = roiset.acc_raw.data[roi.slice]
            self.assertEqual(len(ebb.shape), 4)
            self.assertTrue(np.all([si >= 1 for si in ebb.shape]))
            rbb = ebb[roi.relative_slice]
            self.assertEqual(len(rbb.shape), 4)
            self.assertTrue(np.all([si >= 1 for si in rbb.shape]))

    def test_make_2d_patches(self):
        roiset = self._make_roi_set()
        files = roiset.export_patches(
            output_path / '2d_patches',
            draw_bounding_box=True,
        )
        self.assertGreaterEqual(len(files), 1)

    def test_make_3d_patches(self):
        roiset = self._make_roi_set()
        files = roiset.export_patches(
            output_path / '3d_patches',
            make_3d=True)
        self.assertGreaterEqual(len(files), 1)

    def test_export_annotated_zstack(self):
        roiset = self._make_roi_set()
        file = roiset.export_annotated_zstack(
            output_path / 'annotated_zstack',
        )
        result = generate_file_accessor(Path(file['location']) / file['filename'])
        self.assertEqual(result.shape, roiset.acc_raw.shape)

    def test_flatten_image(self):
        id_map = _get_label_ids(self.seg_mask)

        roiset = RoiSet(self.stack_ch_pa, id_map, params=RoiSetMetaParams(mask_type='boxes'))
        df = roiset.get_df()

        from model_server.base.roiset import project_stack_from_focal_points

        img = project_stack_from_focal_points(
            df['centroid-0'].to_numpy(),
            df['centroid-1'].to_numpy(),
            df['zi'].to_numpy(),
            self.stack,
            degree=4,
        )

        self.assertEqual(img.shape[0:2], self.stack.shape[0:2])

        write_accessor_data_to_file(
            output_path / 'flattened.tif',
            InMemoryDataAccessor(img)
        )

    def test_make_binary_masks(self):
        roiset = self._make_roi_set()
        files = roiset.export_patch_masks(output_path / '2d_mask_patches', )
        self.assertGreaterEqual(len(files), 1)

    def test_classify_by(self):
        roiset = self._make_roi_set()
        roiset.classify_by('dummy_class', 0, DummyInstanceSegmentationModel())
        self.assertTrue(all(roiset.get_df()['classify_by_dummy_class'].unique() == [1]))
        self.assertTrue(all(np.unique(roiset.object_class_maps['dummy_class'].data) == [0, 1]))
        return roiset

    def test_export_object_classes(self):
        record = self.test_classify_by().run_exports(
            output_path / 'object_class_maps',
            0,
            'obmap',
            RoiSetExportParams(object_classes=True)
        )
        opa = record['object_classes_dummy_class']
        self.assertTrue(Path(opa).exists())
        acc = generate_file_accessor(opa)
        self.assertTrue(all(np.unique(acc.data) == [0, 1]))


    def test_raw_patches_are_correct_shape(self):
        roiset = self._make_roi_set()
        patches = roiset.get_raw_patches()
        np, h, w, nc, nz = patches.shape
        self.assertEqual(np, roiset.count)
        self.assertEqual(nc, roiset.acc_raw.chroma)

    def test_patch_masks_are_correct_shape(self):
        roiset = self._make_roi_set()
        patch_masks = roiset.get_patch_masks()
        np, h, w, nc, nz = patch_masks.shape
        self.assertEqual(np, roiset.count)
        self.assertEqual(nc, 1)


class TestRoiSetMultichannelProducts(BaseTestRoiSetMonoProducts, unittest.TestCase):

    def setUp(self) -> None:
        super().setUp()
        id_map = _get_label_ids(self.seg_mask)
        self.roiset = RoiSet(
            self.stack,
            id_map,
            params=RoiSetMetaParams(
                expand_box_by=(128, 2),
                mask_type='boxes',
                filters={'area': {'min': 1e3, 'max': 1e4}},
            )
        )

    def test_multichannel_to_mono_2d_patches(self):
        files = self.roiset.export_patches(
            output_path / 'multichannel' / 'mono_2d_patches',
            white_channel=3,
            draw_bounding_box=True,
        )
        result = generate_file_accessor(Path(files[0]['location']) / files[0]['patch_filename'])
        self.assertEqual(result.chroma, 1)

    def test_multichannnel_to_mono_2d_patches_rgb_bbox(self):
        files = self.roiset.export_patches(
            output_path / 'multichannel' / 'mono_2d_patches_rgb_bbox',
            white_channel=3,
            draw_bounding_box=True,
            bounding_box_channel=1,
        )
        result = generate_file_accessor(Path(files[0]['location']) / files[0]['patch_filename'])
        self.assertEqual(result.chroma, 3)

    def test_multichannnel_to_rgb_2d_patches_bbox(self):
        files = self.roiset.export_patches(
            output_path / 'multichannel' / 'rgb_2d_patches_bbox',
            white_channel=4,
            rgb_overlay_channels=(3, None, None),
            draw_mask=True,
            mask_channel=0,
            rgb_overlay_weights=(0.1, 1.0, 1.0)
        )
        result = generate_file_accessor(Path(files[0]['location']) / files[0]['patch_filename'])
        self.assertEqual(result.chroma, 3)

    def test_multichannnel_to_rgb_2d_patches_contour(self):
        files = self.roiset.export_patches(
            output_path / 'multichannel' / 'rgb_2d_patches_contour',
            rgb_overlay_channels=(3, None, None),
            draw_contour=True,
            contour_channel=1,
            rgb_overlay_weights=(0.1, 1.0, 1.0)
        )
        result = generate_file_accessor(Path(files[0]['location']) / files[0]['patch_filename'])
        self.assertEqual(result.chroma, 3)
        self.assertEqual(result.get_one_channel_data(2).data.max(), 0)  # blue channel is black

    def test_multichannel_to_multichannel_tif_patches(self):
        files = self.roiset.export_patches(
            output_path / 'multichannel' / 'multichannel_tif_patches',
        )
        result = generate_file_accessor(Path(files[0]['location']) / files[0]['patch_filename'])
        self.assertEqual(result.chroma, 5)

    def test_multichannel_annotated_zstack(self):
        file = self.roiset.export_annotated_zstack(
            output_path / 'multichannel' / 'annotated_zstack',
            'test_multichannel_annotated_zstack',
        )
        result = generate_file_accessor(Path(file['location']) / file['filename'])
        self.assertEqual(result.chroma, self.stack.chroma)
        self.assertEqual(result.nz, self.stack.nz)

    def test_export_single_channel_annotated_zstack(self):
        file = self.roiset.export_annotated_zstack(
            output_path / 'annotated_zstack',
            channel=3,
        )
        result = generate_file_accessor(Path(file['location']) / file['filename'])
        self.assertEqual(result.hw, self.roiset.acc_raw.hw)
        self.assertEqual(result.nz, self.roiset.acc_raw.nz)
        self.assertEqual(result.chroma, 1)

class TestRoiSetFromZmask(unittest.TestCase):

    def setUp(self) -> None:
        # set up test raw data and segmentation from file
        self.stack = generate_file_accessor(roiset_test_data['multichannel_zstack']['path'])
        self.stack_ch_pa = self.stack.get_one_channel_data(roiset_test_data['pipeline_params']['segmentation_channel'])
        self.seg_mask_3d = generate_file_accessor(roiset_test_data['multichannel_zstack']['mask_path_3d'])

        id_map = _get_label_ids(self.seg_mask_3d, allow_3d=True)
        self.assertGreater(id_map.nz, 1)

        roiset = RoiSet.from_3d_obj_ids(
            self.stack_ch_pa,
            id_map,
            params=RoiSetMetaParams(
                mask_type='contours',
                filters={'area': {'min': 1e3, 'max': 1e4}},
            )
        )
        self.roiset = roiset
        self.zmask = InMemoryDataAccessor(roiset.get_zmask())

    def test_id_map_connects_z(self):
        id_map = _get_label_ids(self.seg_mask_3d, allow_3d=True)
        labels = np.unique(id_map.data)[1:]
        def _label_is_2d(la): # single label's zmask has same counts as its MIP
            mask_3d = (id_map.data == la)
            mask_mip = mask_3d.max(axis=-1)
            return mask_3d.sum() == mask_mip.sum()
        is_2d = all([_label_is_2d(la) for la in labels])
        print([_label_is_2d(la) for la in labels])
        self.assertFalse(is_2d)

    def test_3d_zmask(self):
        write_accessor_data_to_file(output_path / 'roiset_from_3d' / 'raw.tif', self.roiset.acc_raw)
        write_accessor_data_to_file(output_path / 'roiset_from_3d' / 'ob_ids.tif', self.roiset.acc_obj_ids)
        write_accessor_data_to_file(output_path / 'roiset_from_3d' / 'zmask.tif', self.zmask)